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Subdural Soft Electrocorticography ECoG Array Implantation and Long-Term Cortical Recording in Minipigs
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在头骨窗口成像方案中使用软微电极阵列进行海马体记录:一个验证研究.

G Juhász1, M Madarász2,3, B Szmola2,1

  • 1Two-Photon Measurement Technology Research Group, The Faculty of Information Technology, Pázmány Péter Catholic University, Budapest, Hungary.

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这项研究引入了一种透明的微电极装置,用于同时在小鼠海马中进行两光子成像和电生理学. 该设备能够长期记录神经活动和细胞成像超过六个月.

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科学领域:

  • 神经科学是一个神经科学.
  • 生物医学工程 生物医学工程
  • 光学成像技术的成像

背景情况:

  • 海马对于记忆和导航至关重要.
  • 双光子成像和电生理学是神经科学的关键工具.
  • 慢性体内记录对于理解长期神经过程至关重要.

研究的目的:

  • 开发和验证一种新的透明微电极装置,用于结合体内成像和电生理学.
  • 为了评估设备在清醒,行为良好的小鼠中的长期稳定性和性能.
  • 调查该设备与慢性成像和电生理学记录的兼容性.

主要方法:

  • 在清醒的小鼠中使用软透明微电极 (STM) 装置进行高速两光子海马体成像.
  • 长期植入STM装置在头骨窗口腔内.
  • 同时记录当地现场潜力 (LFP),多单位活动 (MUA) 和单单位活动 (SUA).
  • 监测电极阻抗,用于长期记录质量评估.
  • 在CA1金字塔层中的GCaMP6f标记细胞的成像.
  • 实验后组织学分析使用GFAP染色来评估免疫反应.

主要成果:

  • 该STM装置可从背部海马体进行可靠的大规模电生理记录 (LFP,MUA,SUA) 长达两个月.
  • 在thy1-GCaMP6f转基因小鼠中,GCaMP6f标记细胞的高质量成像在六个多月内实现了.
  • 电极阻抗保持稳定,表明长期记录质量良好.
  • 组织学分析显示,在长期植入后,免疫反应最小.

结论:

  • 开发的透明微电极装置适用于慢性小鼠模型的同时,长期的两光子成像和大规模的电生理学测量.
  • 这项技术为推进神经科学研究提供了有价值的工具,特别是在记忆和导航研究中.
  • 该设备在延长的体内实验中显示出出色的生物相容性和稳定性.