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相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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相关实验视频

Updated: Jun 12, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

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使用单细胞RNA-seqq映射T细胞激活的协议.

Hui Li1, Yifei Liu2, Xuefei Wang1

  • 1CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China.

STAR protocols
|October 20, 2024
PubMed
概括
此摘要是机器生成的。

这项研究详细介绍了一种分析CD4 T细胞激活的协议,使用抗CD3/CD28珠刺激和单细胞RNA测序. 该方法涵盖细胞隔离,刺激和数据分析,用于T细胞激活研究.

关键词:
细胞生物学 细胞生物学细胞隔离细胞的隔离.免疫学 免疫学 免疫学分子生物学分子生物学在RNAseqqq中使用.序列分析是指进行序列分析.测序测序是指测序的时间.一个单细胞单细胞.

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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

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Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
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Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

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相关实验视频

Last Updated: Jun 12, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

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Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
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Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

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科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • CD4 T细胞激活在免疫反应中至关重要.
  • 反CD3/CD28刺激是T细胞激活研究的标准模型.
  • 单细胞RNA测序 (scRNA-seq) 提供了对细胞异质性的高分辨率见解.

研究的目的:

  • 提出研究T细胞激活的综合性方案.
  • 为了进行详细分析,将抗CD3/CD28珠刺激与scRNA-seq结合起来.
  • 为T细胞激活研究提供可复制的工作流程.

主要方法:

  • 隔离人类外周血液单核细胞 (PBMCs).
  • 丰富CD4的T细胞. 丰富CD4的T细胞.
  • 使用抗CD3/CD28珠子进行刺激.
  • 单细胞RNA测序 (scRNA-seq) 和数据分析.

主要成果:

  • 建立了使用scRNA-seq进行T细胞激活分析的详细工作流.
  • 该协议可以在单细胞水平上研究T细胞的反应.
  • 该方法有助于理解T细胞激活过程中的分子变化.

结论:

  • 本方案有效地将抗CD3/CD28刺激与scRNA-seq.
  • 这种方法为剖析T细胞激活机制提供了一个强大的工具.
  • 工作流程支持免疫学研究和治疗开发的进展.