相关实验视频

Updated: Jun 12, 2025

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

26.4K

在哺乳动物细胞中基于CRISPR的内源蛋白标记的协议

Yu-Xi Xiao1, Jiarun Wei1, Jason Moffat2

  • 1Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

STAR protocols
|October 20, 2024
PubMed
概括

这项研究引入了一种基于CRISPR的方法,用于哺乳动物细胞内源性蛋白质标记,使研究人员能够跟踪蛋白质定位和相互作用. 该协议详细标记了特定的人类蛋白质,为功能研究提供了灵活的方法.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?细胞生物学 细胞生物学遗传学 遗传学 是一个高通量选 高通量选显微镜的使用方法分子生物学分子生物学

更多相关视频

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster07:23

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Published on: January 26, 2024

692
Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

9.9K

相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K