在日本使用完全自动化的细菌检测设备RAISUS S4检测抗卡巴因的抗грам阴性细菌
Yumiko Funashima1, Rin Hamabe2, Kei Tominaga2
1Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare, Okawa, Fukuoka, Japan; Medical Laboratory Science Graduate School of Health and Welfare Sciences, International University of Health and Welfare Graduate School, Okawa, Fukuoka, Japan.
Journal of global antimicrobial resistance
|October 20, 2024
概括
这项研究引入了一种快速方法,用于使用meropenem.GNR检测产生卡巴酶的グラム阴性细菌 (CP-GNR). 该RAISUS S4系统表现出高灵敏度和速度,与有效的抗微生物敏感性测试标准方法相对应.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 临床诊断 临床诊断 临床诊断
背景情况:
- 卡巴酶生成的グラム阴性细菌 (CP-GNR) 由于它们对最后手段抗生素的耐药性而构成重大威胁.
- 快速准确地检测CP-GNR对于有效的感染控制和治疗策略至关重要.
研究的目的:
- 用RAISUS S4系统评估使用美罗 (MEPM) 的CP-GNR快速检测方法.
- 评估该方法的效率和与标准抗微生物敏感性测试的相关性.
主要方法:
- 使用RAISUS S4系统监测细菌生长曲线以检测CP-GNR.
- 采用了两种方法:RAISUS 18小时方法和RAISUS快速方法用于肠道细菌 (F-GNR) 和非发酵的阴性菌根 (NF-GNR).
主要成果:
- 使用18小时方法在F-GNR中实现了100%的CP-GNR检测灵敏度,使用快速方法达到98.3%,检测时间短至4.0小时.
- 通过这两种方法,在NF-GNR中检测CP-GNR的灵敏度达到了100%.
- 确定了特定的碳烯酶基因型 (NDM,GES,KPC,IMP,VIM),检测时间不同.
结论:
- RAISUS S4 18-h和快速方法与CLSI微稀释标准具有很高的相关性.
- 该RAISUS S4系统提供了一种快速有效的方法来检测CP-GNR,有助于抗菌敏感性测试.
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