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Modeling Paracrine Noncanonical Wnt Signaling In Vitro
Published on: December 10, 2021
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WNT1/ROR2通路增强了三阴性乳腺癌的入侵,迁移和上皮层-半细胞过渡
Xin Jin1, Chunlan Fu2, Yusa Chen3
1Department of Breast Surgery, Zhuji Affiliated Hospital of Wenzhou Medical University, Zhuj, Zhejiang, China.
Journal of biochemical and molecular toxicology
|October 21, 2024
概括
WNT1/ROR2通路驱动三阴性乳腺癌 (TNBC) 的进展. 在TNBC模型中,用酸 pamoate (PP) 抑制这种途径显示出显著的抗癌效应.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 类似孤儿受体2 (ROR2) 的受体氨酸激酶与各种瘤生长有关.
- 需要进一步阐明WNT1/ROR2信号通路在三阴性乳腺癌 (TNBC) 进展中的作用.
研究的目的:
- 研究WNT1/ROR2信号通路对三阴性乳腺癌 (TNBC) 进展的影响.
- 评估WNT抑制剂酸 pamoate (PP) 对TNBC的治疗潜力.
主要方法:
- 在TNBC组织中对ROR2表达的生物信息学分析.
- 定量逆转录聚合酶连锁反应 (qRT-PCR) 和西部污染 (WB) 来评估ROR2mRNA和蛋白质水平.
- 细胞增殖试验 (Transwell,CCK-8) 和EMT标志物分析.
- 在体外和体外实验中,在TNBC模型中使用酸 pamoate (PP).
主要成果:
- 在TNBC组织中,ROR2表达显著更高,与预后不佳相关.
- 降低ROR2的调节抑制了TNBC细胞的进展和上皮细胞-介质细胞过渡 (EMT).
- 酸 pamoate (PP) 有效抑制了WNT1/ROR2通路,并在TNBC模型中显示出抗癌作用.
结论:
- WNT1/ROR2信号通路促进了三阴性乳腺癌的恶性进展.
- pamoate (PP) 通过抑制这种途径,显示出作为控制TNBC的治疗剂的潜力.
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