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合成酶域在I型多基化合成酶中的非正规功能

Yuqiong Zhao¹, Wenyu Zhang¹, Wen Liu¹

¹State Key Laboratory of Chemical Biology, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, China.

Chembiochem : a European journal of chemical biology

|October 21, 2024

概括

模块化聚基酸合成酶 (PKSs) 使用合成酶 (KS) 域用于超越链延伸的多种化学作用. 本综述探讨了不寻常的KS活动,增强了对PKS组装和工程潜力的理解.

科学领域:

生物化学和分子生物学
自然产品的合成自然产品的合成
酶学是一种酶学.

背景情况:

模块化I型多基化合成酶 (PKSs) 是大型酶复合体,负责合成结构多样化的多基化天然产品.
合成酶 (KS) 域是PKS功能的核心,通常催化脱碳化克莱森凝结,以扩展聚基链.
最近的发现揭示了非正规的KS域在正规凝结之外执行各种催化功能,扩大了PKS已知的功能.

研究的目的:

在模块化I型多基化合成酶 (PKSs) 中,提供由非正规的合成酶 (KS) 域催化的反应的全面概述.
突出这些不寻常的KS领域所表现出的结构多样性和新的催化活动.
为了加深对PKS组装线化学的理解,并为新型聚基化物生成的未来工程工作提供信息.

主要方法:

关于模块化I型PKS和合成酶 (KS) 域活动的已发表研究的文献综述.
分析和分类由非正规的KS领域催化的各种反应.
综合了目前关于PKS原始化,转移,迪克曼凝结,迈克尔添加,阿尔多-乳化,C-N键形成和脱碳化方面的知识.

主要成果:

在I型 PKS中识别和描述多种非正规的 KS 活动,包括原始化,转移,迪克曼凝结,迈克尔添加, aldol-lactonization, C-N 键形成和脱碳化.
证明KS域具有超越传统克莱森凝结的催化多功能性,对多基基结构多样性做出了重大贡献.
证据证明PKS组装线内的KS域具有广泛的基质接受和催化适应性.

结论:

非正规的KS活动显著扩大了模块化I型PKS的化学谱,使复杂的多基基化物合成成为可能.
了解这些不寻常的KS功能对于破译基于PKS的生物合成途径至关重要.
这种知识为PKS的理性工程提供了基础,以创建具有潜在生物活动的新型多基化物天然产品.

关键词:

生物合成生物合成基因合成酶 (ketosynthase) 是一种酶.自然产品是天然产品.非正典的功能功能.第一种类型的多基化合成酶.

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