相关实验视频
Updated: Jul 19, 2026

07:18
Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
11.0K
作为丹丁调节剂的α氧酸对生长因子释放的影响:一个体外研究
Lakshmi Tulasi1, Sihivahanan Dhanasekaran1, Vijay Venkatesh1
1Conservative Dentistry and Endodontics, Sri Ramaswamy Memorial (SRM) Kattankulathur Dental College, Chennai, IND.
Cureus
|October 21, 2024
概括
酸 (CA) 有效地从牙中释放出生长因子. 较温和的酸性或化剂,如CA,酸 (MA) 和乙烯基二胺三酸 (EDTA),可能会促进理想的生长因子效应.
科学领域:
- 生物材料科学 生物材料科学
- 牙科研究 牙科研究
- 分子生物学分子生物学
背景情况:
- 牙调节对于再生牙科至关重要.
- 增长因子在组织再生中起着至关重要的作用.
- 阿尔法氧酸和合剂用于牙调节.
研究的目的:
- 评估阿尔法氧酸和合剂在牙调节中的有效性.
- 评估这些药物对生长因子释放的影响.
- 确定促进生长因子中介效应的最佳药物.
主要方法:
- 人类牙切片用17%的乙烯基胺三酸 (EDTA),10%的甘油酸 (GA),10%的酸 (CA) 和5%的酸 (MA) 进行5分钟和10分钟的调节.
- 转化生长因子β-1 (TGF-β1) 释放量通过酶相关免疫吸收试验 (ELISA) 来量化.
- 还测量了牙表面TGF-β1的水平.
主要成果:
- 酸 (CA) 在经过5分钟的训练 (p<0.05) 后,在释放生长因子方面表现最为有效.
- 与其他药物相比,糖醇酸 (GA) 处理导致TGF-β1的表面水平显著降低.
- 该研究确定了最佳生长因子释放的特定代理和持续时间.
结论:
- 较温和的酸性或化剂,包括CA,MA和EDTA,建议用于牙调节.
- 这些药物可以促进可取的生物生长因子介导作用.
- 这些发现支持使用特定的调节剂来增强再生潜力.
相关概念视频
Allosteric Regulation
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Regulation of Metabolism
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Hormones and Bone Tissue
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Factors Influencing Microbial Growth: pH
Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
Designing Growth Media for Bioreactors
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

