具有多环形异oprenoid 部分的核糖体.
Florian Hubrich1,2, Sanath K Kandy3, Clara Chepkirui1
1Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, Vladimir-Prelog Weg 4, 8093 Zürich, Switzerland.
概括
科学家们发现了使用复杂的循环单元来修改的新方法,这些单元对于它们的抗菌活性至关重要,并且具有广泛的生物技术潜力.
科学领域:
- 生物化学 生物化学
- 自然产品 化学 化学
- 分子生物学分子生物学
背景情况:
- 异oprenoid 修饰 (prenylation) 对蛋白质和的功能至关重要,其中 farnesyl 和 geranylgeranyl 是常见的.
- 核糖体前化通常涉及比蛋白质中发现的更小的异oprenoid 单元.
- 生物分子中循环烯修饰以前没有报道过.
研究的目的:
- 识别具有独特异oprenoid 修饰的新型核糖体合成和翻译后修饰 (RiPPs).
- 调查负责安装这些复杂修改的酶.
- 探索这些改性的生物活性和生物技术应用.
主要方法:
- 针对性基因组挖掘,以确定相关的基因集群.
- 在合适的宿主生物体中异质途径的复制.
- 双功能成熟酶 (prenyltransferase-terpene cyclase融合) 的生化特征.
- 改性产品的结构阐明.
- 生物测试用于评估生物活性.
主要成果:
- 发现了具有复杂,循环基拉尼尔基拉尼尔修饰的RiPPs,这是以前未报告的修饰类型.
- 鉴定了新的双功能成熟酶与融合的前转移酶和烯环酶域.
- 一种带有伪固醇结合的多环结构的蓝藻细菌蛋白质素的表征.
- 证明异oprenoid 修饰对于观察到的适度抗菌活性是必不可少的.
结论:
- 这项研究揭示了一种新的循环化异oprenoid-modified (RiPPs) 类.
- 已识别的成熟酶具有独特的化前转移酶-烯循环酶架构.
- 这些发现扩大了已知的自然产品修改作品集,并表明在生物技术中具有广泛的适用性.
- 在不同的RiPP家族中预测了周期性异oprenoid单位,突出显示了该系统的多功能性.
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