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对菌 (Enterococcus gilvus) 硫糖溶解的研究揭示了二基硫酸盐降解的广泛分叉途径

Yiwei Chen¹, Ruoxing Chu^2,3, Kailiang Ma^2,3

¹College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

|October 21, 2024

概括

在Enterococcus gilvus中,一种新的硫基诺 (SQ) 降解途径产生了3-基硫酸盐 (3-HPS) 和3-硫酸盐 (3-SP). 3-SP是Bilophila wadsworthia的呼吸电子受体,产生有毒的硫化.

科学领域:

微生物学微生物学
生物化学生物化学
代谢途径代谢途径

背景情况:

硫基诺 (SQ) 是硫脂的关键成分,对于光合作用至关重要.
无氧固醇通过硫-转酶 (硫-TAL) 路径降解SQ,产生二基硫酸盐 (DHPS).
一些细菌进一步将DHPS代谢为3-基硫酸盐 (3-HPS).

研究的目的:

为了阐明Enterococcus gilvus中的一种新的硫-TAL路径变体.
为了确定这种替代代代谢途径的酶和产物.
研究这些代谢物对微生物交叉养和肠道健康的影响.

主要方法:

基因组分析以识别硫-TAL通路中的新基因.
在SQ和DHPS上种植Enterococcus gilvus.
代谢物分析以确定3-HPS和3-硫胺 (3-SP).
使用Bilophila wadsworthia与3-HPS和3-SP进行呼吸试验.

主要成果:

杆菌使用分叉的硫-TAL通路,产生3-HPS和3-SP.
该途径涉及一种新的NAD+依赖的3-SPA脱酶 (HpfX) 和3-硫烯-CoA合成酶 (HpfYZ).
3-SP,但不是3-HPS,作为Bilophila wadsworthia的呼吸电子受体,产生有毒的H2S.

结论:

一种新的硫-TAL路径变异扩展了已知的SQ代谢路径.
这一途径有助于硫酸盐在无氧微生物群中的交叉食相互作用.
3-SP的产生对病原生物活动和肠道中硫化生成有影响.

关键词:

生物科学生物科学微生物的新陈代谢微生物学微生物学

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