激活转录因子6在试验性静脉瘤诱导的流行性上皮细胞中调解炎症
Yin-Shan Jin1, Yuan-Qing Cui1, Yan-Ping Xu1
1Department of Reproductive Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, People's Republic of China.
Journal of inflammation research
|October 21, 2024
概括
静脉淋巴细胞通过损害皮层细胞导致男性不孕. 在这种情况下,激活转录因子6 (ATF-6) 被激活,这表明ATF-6通路的抑制是治疗瘤诱导不孕症的潜在疗法.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 男人不孕症的常见原因是静脉,涉及内部精子静脉的扩张.
- 脊髓上皮细胞在精子的成熟和功能中起着至关重要的作用.
- 活化转录因子6 (ATF-6) 在瘤诱导的脑膜外皮损伤中的作用尚不清楚.
研究的目的:
- 调查ATF-6在试验性静脉瘤诱导的外皮膜上皮细胞损伤中的作用.
- 探索向ATF-6通路作为治疗瘤的治疗策略的潜力.
主要方法:
- 在实验中,通过部分左静脉绑定,在老鼠中诱导了静脉.
- 使用H&E和TUNEL染色来评估流行病损伤.
- 测量了NAG,ATF-6,TNF-α和p-NF-κB p65的表达;ATF-6在体外使用siRNA沉默,细胞暴露于缺氧.
主要成果:
- 静脉瘤诱导了外皮膜上皮细胞缩和亡,降低了NAG表达.
- ATF-6,TNF-α和p-NF-κB p65的表达在受瘤影响的脊髓炎患者中升高.
- 在接受过氧治疗的细胞中,ATF-6沉默提高了活力,并降低了TNF-α,IL-8和p-NF-κB p65水平.
结论:
- 在实验中,ATF-6通路被激活在瘤诱导的脑膜外皮损伤.
- 抑制ATF-6通路可能为与静脉相关的 epididymal 功能障碍提供一种新的治疗方法.
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