人类ESC衍生的外胚细胞与小鼠胚胎细胞的合体化
Siyi Fu1, Borong Huang1, Enqin Li1
1Center of Reproduction, Development & Aging, and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
International journal of biological sciences
|October 21, 2024
概括
人类胚胎干细胞衍生的胚胎外细胞可以在老鼠嵌合体中形成胎盘和软骨组织. 这些发现表明了胚外细胞的发育潜力,并为其研究提供了新的方法.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 生殖生物学 生殖生物学
背景情况:
- 人类胚胎干细胞 (hESCs) 可以在体外使用特定的生长因子和抑制剂分化为胚外细胞 (ExE).
- 伦理限制限制了使用人类胚胎对人类ExE细胞发育潜力的体内研究.
研究的目的:
- 为了研究hESC衍生的ExE细胞的体内发育潜力.
- 建立研究ExE细胞分化和对胚胎和胚胎外组织的贡献的新方法.
主要方法:
- 使用BMP4,A83-01和PD173074 (BAP) 的方法将hESC分化为ExE细胞.
- 将ExE细胞移植到小鼠子宫和胚胎囊中.
- 化学胚胎被分析为人类细胞贡献和标记物表达在E14.
主要成果:
- 由hESC衍生的ExE细胞表达了热囊细胞和羊水细胞的标记物.
- 宫内移植显示了ExE细胞对小鼠胎盘的贡献.
- 胚胎内注射导致了ExE细胞嵌合体,有助于胎盘和胚胎软骨 (SOX9+).
- 删除MSX2限制了ExE细胞对胎盘的贡献.
结论:
- 由hESC衍生的ExE细胞可以与小鼠胚胎囊细胞合并,为胎盘和胚胎组织做出贡献.
- ExE细胞表现出异质的发育潜力,包括中皮分化.
- 宫内和母细胞内注射是研究ExE细胞发育潜力的有效方法.
相关概念视频
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...