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通过使用三旋探针进行硫交换反应,同时定量硫化物,生物醇和硫

Xiaoli Tan¹, Jiaxin Zhou¹, Luhua Yang¹

¹The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China.

Journal of the American Chemical Society

|October 21, 2024

概括

新的电子磁共振 (EPR) 探测器可以精确检测和量化反应硫物种 (RSS),包括硫化物和生物醇. 这一突破可以同时测量多个RSS, 进一步了解它们的生理作用.

科学领域:

分析化学
生物化学
光谱学

背景情况:

反应硫种 (RSS),如化物 (RSSH),生物乙醇和硫化 (H2S),对于许多生理功能至关重要.
了解这些RSS之间的复杂相互作用和转变至关重要,但由于缺乏精确的分析方法而受到阻碍.
在生物化学研究中,开发用于区分检测和定量各种RSS的技术是一个重大挑战.

研究的目的:

设计和合成新型电子磁共振 (EPR) 探针,用于敏感和选择性检测活性硫种.
建立一种光谱方法,同时量化多种RSS,包括化物,生物醇和硫化.
应用这些新型探测器来测量生物样本中的RSS水平,例如分离的小鼠肝脏.

主要方法:

基于三基的EPR探针的合理设计和合成 (CT02-TNB和OX-TNB).
使用EPR探测器和目标RSS之间的硫交换反应来产生信号.
使用EPR光谱和光谱模拟进行检测和量化.
在生物矩阵中的现场测量中应用性OX-TNB.

主要成果:

开发的EPR探针CT02-TNB和OX-TNB显示了与各种RSS的快速和特定反应,包括两种不同的化物 (PS1,PS2),化物 (GSH),化物 (Cys),H2S和硫酸盐.
对结合物获得了特征性的EPR光谱,使不同RSS的区分检测和定量成为可能.
使用CT02-TNB和更新的EPR光谱模拟程序实现了多个RSS (例如PS1/GSH/Cys或PS1/GSH/H2S) 的同时定量.
水性探针OX-TNB成功测量了分离的小鼠肝脏中释放的PS1和内生GSH水平.

结论:

这项研究提出了第一个能够分辨和定量检测各种硫化物和其他活性硫的光谱方法.
这种新型的EPR探测器为进一步了解RSS代谢及其生理意义提供了强大的工具.
开发的方法对未来涉及RSS的氧化还原信号和疾病机制的调查具有前景.

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