响应H2S的转录因子ERF.D3调节番茄酸代谢,叶子衰老和水果成熟
Kangdi Hu1, Meihui Geng1, Lin Ma1
1School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Plant physiology
|October 21, 2024
概括
硫化 (H2S) 通过调节ERF.D3蛋白来延缓番茄的叶子衰老. 这种对H2S有反应的因素,ERF.D3,影响酸 (ABA) 水平,并影响水果成熟.
科学领域:
- 植物生物学 植物生物学
- 分子植物生理学分子植物生理学
- 信号分子的信号分子
背景情况:
- 硫化 (H2S) 作为植物中的关键信号分子,影响各种生理过程.
- 植物衰老是一个复杂的发育过程,受到多种内部和外部因素的严格调节.
- 了解衰老背后的分子机制对于农业应用至关重要,例如延长作物新鲜度.
研究的目的:
- 为了研究H2S在调节番茄叶的暗感诱导衰老中的作用.
- 识别和描述参与衰老和果实成熟的H2S反应因素.
- 阐明H2S,ERF.D3和酸 (ABA) 代谢之间的分子相互作用.
主要方法:
- 转录组分析和RT-qPCR用于识别H2S响应基因.
- CRISPR/Cas9基因编辑和基因过度表达以评估ERF.D3功能.
- 生物化学试验包括化分析,双化酶记者试验和电泳性移动性转换试验 (EMSA).
主要成果:
- 发现H2S可以延迟番茄叶的暗感诱导衰老.
- ERF.D3被确定为一个关键的H2S反应因子,对叶子衰老和果实成熟产生负面调节.
- ERF.D3直接向CYP707A2,这是一种参与ABA降解的基因,其化增强了这种活性.
- 通过RNF217介导的ERF.D3的泛化可能会影响后期发育阶段的水果成熟.
结论:
- ERF.D3是H2S信号的关键媒介,可以延迟番茄叶的衰老和果实成熟.
- 由H2S诱导的ERF.D3的持续硫化增强了其在CYP707A2上的转录活性,将H2S与ABA降解联系起来.
- 这项研究揭示了一种涉及H2S,ERF.D3和ABA代谢的新型调节途径,用于控制植物衰老和成熟.
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