植物生长的变化和生物合成活动的Calendula officinalis毛发根
Nadiia Matvieieva1, Taisa Bohdanovych1, Valeriia Belokurova1
1Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Preparative biochemistry & biotechnology
|October 21, 2024
概括
Calendula officinalis毛的根,通过Agrobacterium rhizogenes的转化产生,积累了具有抗氧化特性的显著黄类. 甲基斯酸盐增强了黄化合物合成和抗氧化活性,使得银纳米粒子的生产.
科学领域:
- 植物生物技术 植物生物技术
- 药用化学 医学化学
- 纳米技术 纳米技术
背景情况:
- 香 (Calendula officinalis) 是一种富含药理学价值的二次代谢产物药用植物.
- 无菌体培养,特别是毛发根,是生物活性化合物生产的有希望的来源.
- 来自C. officinalis的黄酸具有显著的抗氧化活性.
研究的目的:
- 建立一个高频率的Agrobacterium rhizogenes介导的转化系统,用于Calendula officinalis毛的根.
- 为了研究甲基斯酸盐对毛发根生长,黄类积累和抗氧化活性的影响.
- 探索C. officinalis毛发根提取物在银纳米粒子合成中的潜力.
主要方法:
- 通过Agrobacterium rhizogenes介导的Calendula officinalis的遗传转化.
- 培养毛的根系和评估生长速度.
- 量化黄酸含量和评估抗氧化活性 (减少功率).
- 使用植物提取物优化提取条件和合成银纳米颗粒.
主要成果:
- 实现了百分之百的Calendula officinalis毛发根诱导频率.
- 毛发根线表现出不同的生长速度和黄类积累,达到6.68mg/g (湿重).
- 甲基斯蒙酸盐 (10μM) 轻微抑制了生长,但显著刺激了黄类化合物合成和增强了抗氧化活性.
- 观察到黄酸含量与抗氧化剂/降解功率之间存在强烈的相关性.
- 毛发根的提取物表现出强大的减少活性,促进了银纳米颗粒的合成,由TEM证实.
结论:
- Agrobacterium rhizogenes是产生Calendula officinalis毛发根的有效工具,用于生产生物活性化合物.
- 甲基斯酸盐是一种强大的诱导剂,可以增强C. officinalis毛发根中的黄类生物合成和抗氧化特性.
- 卡伦杜拉 (Calendula officinalis) 毛的根提取物具有显著的抗氧化和减少能力,适合纳米颗粒的绿色合成.
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