在简单疹病毒的潜伏期内,疹促进剂活性取决于基因组位置
Navneet Singh1, Sherin Zachariah1, Aaron T Phillips1
1John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
Journal of virology
|October 21, 2024
概括
简单疹病毒1 (HSV-1) 溶性基因促进体可以在延迟期间活跃,但只有当它位于延迟相关转录 (LAT) 位点附近时才会活跃. 这表明,在潜伏期间的病毒基因调节受到基因组接近活性区域的影响.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 简单疹病毒1 (HSV-1) 确立了终身潜伏感染.
- 延迟传统上被视为静止状态,但证据表明零星的lytic基因表达.
- 之前的研究使用CRE标记模型检测了在延迟期间的Lytic基因促进器活性.
研究的目的:
- 调查在潜伏期内外宫和本地HSV-1光性基因促进者的活性.
- 为了确定促进子位置是否会影响潜伏期间的Lytic基因表达.
- 评估活动是否可以在促进体的自然基因组环境中检测到.
主要方法:
- 在HSV-1感染的小鼠模型中使用了敏感的Cre-marking模型.
- 在各种基因组位置检查了异胎性性基因促进体的CRE表达.
- 在自然环境中评估了来自本地性基因促进者的Cre表达.
主要成果:
- 在乳房外和本地HSV-1性基因促进体的潜伏期间检测到Cre表达.
- 这种可检测的活性仅限于位于独特的长基因组段末端附近的促进体.
- 这个基因组区域靠近延迟关联转录 (LAT) 位点.
结论:
- 本地HSV-1性基因促进体可以在延迟期间产生蛋白质产品.
- 在潜伏期内检测到基因促进活性取决于基因组位置,特别是接近LAT位置.
- 这些发现影响了对HSV-1在潜伏期的基因调节的理解,以及转录活性区域对邻近的病毒DNA的影响.
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