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麻疹病毒的集体传染单位导致致命的人类大脑疾病包括许多局部受限和少数广泛复制的缺陷基因组

Biruhalem Taye¹, Iris Yousaf^1,2, Chanakha K Navaratnarajah^1,2

¹Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, USA.

Journal of virology

|October 21, 2024

概括

在麻疹病毒患者的大脑中发现了复制后缺陷的病毒基因组 (cbDVGs). 这些病毒片段可能已经被积极选择,并可能影响疾病,但它们的确切作用需要进一步研究.

科学领域:

病毒学病毒学
神经科学是一个神经科学.
免疫学免疫学免疫学

背景情况:

已知反复复制有缺陷的病毒基因组 (cbDVGs) 在体外诱导先天免疫反应.
它们在人类自然感染,特别是大脑中的存在和影响尚不清楚.
亚急性硬化大脑炎 (SSPE) 是麻疹病毒 (MeV) 感染的罕见,致命的神经并发症.

研究的目的:

调查SSPE患者大脑中的cbDVGs的存在和多样性.
为了确定cbDVGs是否是集体感染单位 (CIU) 的一部分,驱动MeV在大脑中的传播.
探索cbDVGs对MeV病原和宿主免疫反应的潜在影响.

主要方法:

使用下一代测序分析SSPE患者的大脑样本的分析.
对cbDVG物种的识别和量化.
转录组分析以评估宿主基因表达,包括干扰素和炎症反应.
将cbDVG分布与全长MeV基因组进行比较.

主要成果:

在患者的大脑中发现了270多种不同的复制能力强的cbDVG物种.
在多个大脑部位发现了6种cbDVG物种,这表明与CIU长途旅行.
cbDVG与全长MeV基因组的比率通常接近1.
虽然干扰素和炎症基因被上调,但它们的水平与cbDVG丰富度没有相关.
该MeV CIU包括众多局部cbDVGs和一些广泛分布的物种.

结论:

负责该患者SSPE病变的MeV CIU包含各种cbDVG物种,以及全长基因组.
广泛的cbDVG物种可能在大脑中被积极选择.
这些cbDVG在SSPE病变发生过程中的确切作用尚待阐明.

关键词:

大脑转录组转录组是大脑的转录组.大脑的热带主义大脑的热带主义集体传染病单位集体传染病单位回复复本有缺陷的病毒基因组.有缺陷的干扰干扰.有缺陷的病毒基因组.人类大脑人类大脑天生的免疫反应.麻疹病毒的病毒亚急性硬化大脑炎 (SCPA)

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