一个蛋白质组学管道用于从数据独立获取质谱法 (DIA-MS) 发现中生成临床级生物标志物候选物
Qin Fu1, Manasa Vegesna1, Niveda Sundararaman1
1Cedars Sinai Medical Center, Los Angeles, CA, USA.
Angewandte Chemie (International ed. in English)
|October 21, 2024
概括
我们开发了有针对性的提取量化评估 (TEAQ) 软件,以改善使用质谱 (MS) 发现临床生物标志物的蛋白质量化. 为了更快的临床转化,TEAQ简化了针对性测试的开发.
科学领域:
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
- 生物技术是生物技术.
背景情况:
- 临床生物标志物开发面临的挑战是从质谱 (MS) 发现数据中不准确量化蛋白质.
- 生物标志物的验证过程往往是漫长和低效的.
研究的目的:
- 引入有针对性的提取量化评估 (TEAQ) 软件包.
- 为了简化从发现数据中选择可靠的前体,和蛋白质,用于有针对性的测试.
主要方法:
- TEAQ使用来自发现队列的数据独立获取 (DIA) 分析.
- 在高分辨率精确质量谱仪 (HRAM) 平台上,前体被评估以分析标准,包括线性,特异性,可重复性和可重复性.
- 对来自炎症性肠病队列 (n=492) 的血样本进行了分析.
主要成果:
- 在炎症性肠病队列中的1179个已识别的蛋白质中,TEAQ确定了1110个可量化的蛋白质的326个签名.
- 该软件包有助于选择符合针对性测试严格分析标准的和蛋白质.
- 生成的数据可以作为开发其他有针对性的测试的资源.
结论:
- 将TEAQ分析应用于发现数据显著简化了有针对性的测试开发.
- 这种方法促进了从发现到验证和临床研究的更顺的过渡.
- 使用质谱测量,TEAQ提高了临床生物标志物开发的准确性和效率.
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