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Updated: Jun 9, 2025

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
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Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

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鼠标角膜免疫细胞异质性通过单细胞RNA测序揭示

Ebru Yaman1, Nicole Heyer1, Cintia S de Paiva1

  • 1Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States.

Investigative ophthalmology & visual science
|October 21, 2024
PubMed
概括

这项研究揭示了小鼠角膜中的多样化的免疫细胞格局,识别了新的巨细胞亚型和基于性别的基因表达差异. 空间映射证实了免疫细胞的分布,增强了对角膜免疫的理解.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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