Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
Sanger Sequencing01:57

Sanger Sequencing

753.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
753.1K
Next-generation Sequencing03:00

Next-generation Sequencing

87.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.7K
Genomics02:02

Genomics

36.1K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.1K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K
Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Flight style and metabolism shape the tempo of genome evolution in birds.

PLoS biology·2026
Same author

Context-aware simulation enables systematic optimization of long-read mapping parameters.

GigaScience·2026
Same author

Optimizing Treatment Decision Estimation for Right-Censored Survival Data Through Parameter Transfer Learning.

Statistics in medicine·2026
Same author

A comprehensive reference genome assembly dataset of birds inhabiting Denmark, Greenland, and the Faroe Islands.

GigaScience·2026
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Targeting PI3Kγ/AKT1-OCT4/SOX2 axis with eganelisib suppresses tumor growth and prevents ovarian failure in malignant ovarian germ cell tumors.

The international journal of biochemistry & cell biology·2026

相关实验视频

Updated: Jun 9, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K

GCI:一个完整基因组组装的连续性检查员.

Quanyu Chen1,2,3, Chentao Yang4,5, Guojie Zhang1,2,6

  • 1International Institutes of Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 322000, China.

Bioinformatics (Oxford, England)
|October 21, 2024
PubMed
概括

一个新的工具,基因组连续性检查器 (GCI),以单核酸分辨率评估基因组组装连续性. GCI提供分数来评估组件与端粒对端粒 (T2T) 标准的接近程度,解决传统指标的局限性.

更多相关视频

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

9.5K

相关实验视频

Last Updated: Jun 9, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

9.5K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 长期读取的测序进步使高质量的基因组组装成为可能.
  • 端粒对端粒 (T2T) 无间隙组装是新的标准.
  • 像N50这样的现有指标对于T2T评估是不够的.

研究的目的:

  • 为评估T2T基因组组装质量制定一个通用标准.
  • 创建一个单核酸解析工具,用于评估基因组连续性.
  • 为了解决在完整基因组时代准确无间隙评价的需要.

主要方法:

  • 引入了基因组连续性检查器 (GCI) 工具.
  • 使用多个对齐器将长读数映射到组件中.
  • 包含高可靠性读取对齐的精心策划的映射覆盖.

主要成果:

  • 在单基底分辨率下,GCI评估了基因组组装连续性.
  • GCI评估了接近T2T级别组装的情况.
  • GCI 识别了潜在的组装问题,并用 GCI 评分量化了连续性.

结论:

  • GCI提供了一种强大的方法来评估基因组组装连续性.
  • 该工具有助于区分真正的T2T组件和接近T2T的组件.
  • GCI为评估全基因组质量提供了可靠的指标.