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松酸介导的聚胺氧化酶6驱动了草食动物诱导的米中的聚胺催化作用

Hongyue Zu¹, Jing Zhang², Weiwei Bai^1,3

¹State Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.

The Plant journal : for cell and molecular biology

|October 21, 2024

概括

米植物使用聚胺氧化酶 (PAO) 来防御害虫. OsPAO6调节精氨酸水平,影响棕色植物的食和植物防御.

科学领域:

植物生物学植物生物学
生物化学生物化学
昆虫学昆虫学是一门学科.

背景情况:

多氨基酸 (PA) 对于植物对环境压力的防御至关重要.
含有黄素的聚胺氧化酶 (PAO) 通过终端氧化控制PA水平.
在植物-草食动物相互作用中PAO的功能尚不清楚.

研究的目的:

调查OsPAO6在防治棕色虫 (BPH) 侵袭的米防御中的作用.
为了阐明在草食动物攻击期间OsPAO6的调节机制.

主要方法:

在CRISPR-Cas9基因组编辑中,在大米中产生PAO6突变.
在对BPH感染的反应中分析PA水平和合的PA.
对OsPAO6及其促进区的转录分析.
调查斯酸盐 (JA) 信号和MYC2.2的作用.

主要成果:

BPH 感染破坏了 PA 稳态,并诱导了米叶盖中的 OsPAO6.
在BPH攻击后,pao6突变体显示出高水平的精素和联基精素水平.
pao6突变体经历了增加的BPH养,蜂蜜露分泌和植物损伤.
OsPAO6转录通过JA信号通过MYC2与其促进体结合来积极调节.

结论:

OsPAO6通过调节PA代谢,在防治BPH的米中发挥着重要作用.
OsPAO6是米中草食动物攻击的莉花诱导反应的关键组成部分.
由OsPAO6影响的精氨酸水平直接影响BPH养行为.

关键词:

棕色的植物 (Nilaparvata lugens) 是一种雅斯蒙酸是一种酸.聚胺的催化作用聚胺氧化酶6的作用米 (Oryza sativa) 是一种大米.精子中的精子.

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