非电活性细菌在使用电场的AnMBR生物膜控制中表现不同
Lijie Zhou1, Fei Wu1, Pingxiang Ou1
1College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, PR China.
Water research
|October 21, 2024
概括
非电活性细菌在电场控制的厌氧膜生物反应器 (AnMBRs) 中对生物膜形成起着至关重要的作用. 一个0.5伏电场极大地增强了它们的活动,导致了显著的生物膜发育和改变了微生物社区结构.
科学领域:
- 微生物学 微生物学
- 环境工程 环境工程
- 生物技术是生物技术.
背景情况:
- 已知电活性细菌在厌氧膜生物反应器 (AnMBR) 中对电场做出反应.
- 在AnMBR系统中,尽管在生物膜中存在非电活性细菌,但它们在很大程度上被忽视.
- 了解所有微生物群体的作用对于优化AnMBR性能至关重要.
研究的目的:
- 研究不同低压电场下的AnMBR中非电活性细菌的作用和影响.
- 确定影响非电活性细菌活动和生物膜形成的关键电场条件.
- 阐明导致非电活性细菌对生物膜发育贡献的分子机制.
主要方法:
- 来自E-AnMBR (电场辅助AnMBR) 的生物膜的培养和分析在0,0.3,0.5和1V下运行.
- 微生物社区结构分析.
- 评估生物膜的形成速度和特性 (蛋白质/多糖比,泽塔潜力).
- 基因表达与代谢途径和定数感应相关的基因表达分析.
主要成果:
- 在所有测试电压中都存在非电活性细菌 (蛋白质细菌,细菌菌体,chloroflexi).
- 0.5V的临界电压显著增强了非电活性细菌的生长和生物膜的形成 (0.61kPa/天).
- 0.5V场独特地改变了微生物群落结构,提高了碳代谢和氧化酸化基因的调节,并增加了生物膜的蛋白质/多糖合物比率和zeta潜力.
- 观察到对定数感应基因的升级,促进了协调的细菌活动.
结论:
- 非电活性细菌在E-AnMBR中具有重要的功能,特别是在特定的电场条件下.
- 0.5V电场被确定为一个关键参数,通过刺激非电活性细菌来促进显著的生物膜发育.
- 电场可以通过定量检测和代谢途径调节AnMBR中的微生物社区动态和生物膜特征.
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