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通过METTL3/FAM155B轴,CircABCC1可以降低子宫内膜受体

Lan Luo¹, Mi Tang¹, Licen Xie¹

¹Department of TCM Gynecology, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, P.R. China.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

|October 21, 2024

概括

循环RNA circABCC1通过调节FAM155B表达来抑制子宫内膜受体性. 这一发现为胚胎植入失败和复发植入失败 (RIF) 提供了新的见解.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
细胞生物学细胞生物学

背景情况:

子宫内膜受体受损是胚胎植入失败的主要原因.
圆形RNAs (circRNAs) 在子宫内膜受体性中的作用在很大程度上仍未被探索.
复发性植入失败 (RIF) 在辅助生殖技术中是一个重大挑战.

研究的目的:

研究circABCC1在调节子宫内膜受容性的作用.
为了阐明circABCC1作用的潜在分子机制.
为了确定RIF的潜在生物标志物.

主要方法:

在GEO数据库中选RIF生物标志物.
用circABCC1和FAM155B载体培养和移植子宫内膜上皮细胞 (EECs).
评估细胞增殖 (CCK-8),受体因子 (LIF,DKK1通过西方抹杀和ELISA),以及 trofhoblast粘附在体外.
使用RNA免疫沉降 (RIP) 和m⁶A修饰分析 (MeRIP-qPCR) 调查相互作用.

主要成果:

在RIF患者中,CircABCC1和FAM155B的上调.
过度表达circABCC1或FAM155B降低了EEC增殖,LIF/DKK1表达,LIF分泌和 trofhoblast粘附.
通过METTL3-介导的m⁶A修改,CircABCC1对FAM155B表达进行了积极调节.
FAM155B部分挽救了circABCC1淘汰的影响.

结论:

CircABCC1与METTL3相互作用,以调节FAM155BmRNA的修饰和表达.
这种机制抑制了EEC的增殖,并降低了子宫内膜受体,从而导致RIF.
CircABCC1和FAM155B是改善子宫内膜接受力的潜在治疗点.

关键词:

宫内膜的受体性宫内膜的受体性这就是FAM155B.在METTL3中,METTL3是METTL3的第一个类型.在CircABCC1中使用.m6A 修改的修改

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