1电梯运输周期受到自抑制和激活的约束
Yan Jiang1,2, Jiansen Jiang3
1Laboratory of Membrane Proteins and Structural Biology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. yan.jiang@sydney.edu.au.
Nature communications
|October 21, 2024
概括
植物酸盐运输体 (BORs) 如阿拉比多普西斯塔利亚纳Bor1 (AtBor1) 需要精确的监管. 这项研究揭示了AtBor1
科学领域:
- 植物生物学 植物生物学
- 分子机制的分子机制
- 生物化学 生化学
背景情况:
- 对植物生长至关重要,但在高度下是有毒的.
- 酸盐运输体 (BORs),同类于SLC4家族,调节的吸收.
- 1 (AtBor1) 呈现缓慢调节;快速调节机制是未知的.
研究的目的:
- 为了阐明阿拉比多普西斯莉亚的Bor1 (AtBor1) 载体快速调节的分子机制.
- 揭示AtBor1活动调节的结构基础.
主要方法:
- 低温电子显微镜 (cryo-EM) 用于确定高分辨率结构.
- 现场定向突变发生,以调查特定残留物.
- 功能性特征测试用于评估传送器活动.
主要成果:
- 在无活性和活性状态下获得了AtBor1的高分辨率结构.
- 卡基末端的一个自抑制域通过盐桥阻断了基质的接入.
- Thr410的酸化使其能够与充电的口袋相互作用,从而促进酸盐的运输.
结论:
- AtBor1的活性是由自抑制域和Thr410.10的酸化调节的.
- 这些机制使得植物中的酸盐运输能够快速调节.
- 这些发现为植物的恒温提供了分子洞察力.
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