从发酵大米中分离出来的Lactiplantibacillus pentosus的基因组特征和益生菌性质
Athira Cheruvari1,2, Rajagopal Kammara3,4
1Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, 570020, India.
Probiotics and antimicrobial proteins
|October 21, 2024
概括
这项研究描述了发酵大米中的Lactiplantibacillus pentosus (krglsrbmofpi2),揭示了益生菌的潜力. 该菌株对主要病原体表现出强烈的对抗活性,这表明食品工业的应用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 食品科学 食品科学 食品科学
背景情况:
- 传统发酵食品是有益微生物的来源.
- 乳植物菌 (Lactiplantibacillus pentosus) 是一种乳酸细菌,具有已知的益生菌性质.
- 新型菌株的表征对于识别新的益生菌至关重要.
研究的目的:
- 从发酵大米中进行一个Lactiplantibacillus pentosus菌株 (krglsrbmofpi2) 的初步遗传和表型表征.
- 评估已识别的菌株的安全性和益生菌潜力.
- 评估其对优先病原体的对抗活性.
主要方法:
- 全基因组测序和生物信息学分析.
- 生物化学和生物物理分析 (自聚,协聚,疏水性,抗氧化活性,胃肠耐受性).
- 通过血液溶解活性测试进行安全评估.
- 对世界卫生组织优先病原体进行抗微生物活性测试.
主要成果:
- 这种L. pentosus菌株krglsrbmofpi2拥有3.7Mb的基因组,具有3192个蛋白质编码序列.
- 生物信息分析确定了与菌体,等离子体,致病性,抗生素耐药性和细菌素相关的基因.
- 该菌株表现出显著的自聚,协聚,抗氧化活性和对模拟胃肠道疾病的耐受性.
- 它对Vibrio cholerae,Clostridium perfringens,Salmonella Typhi,Escherichia coli,Listeria monocytogenes和Staphylococcus aureus表现出强烈的对抗性活性. 这种药物具有强烈的抗性作用.
- 没有观察到血液溶解活性,这表明安全.
结论:
- 乳植物菌种pentosus菌株krglsrbmofpi2显示出有前途的益生菌特性,包括广泛的抗菌活性.
- 它的遗传和功能性质要求进一步研究其在食品工业中作为益生菌的潜在用途.
- 这种菌株代表了开发功能性食品和益生菌的潜在候选人.
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