查差异表达蛋白质以区分胸膜瘤 (B1和B3) 和胸膜囊,基于双重质量标记 (TMT) 技术
Jingwei Shi1, Rusong Yang1, Xin Chen2
1Department of Thoracic Surgery, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.
Journal of cardiothoracic surgery
|October 21, 2024
概括
鉴定胸膜囊和胸膜瘤的生物标志物至关重要. 甲状腺瘤中甲状腺素-3结合蛋白 (LGALS3BP) 的高调显著,这表明它是潜在的诊断生物标志物.
科学领域:
- 在瘤学瘤学.
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 对胸膜囊的治疗策略进行了辩论.
- 准确的诊断胸膜囊与胸膜瘤 (THYM) 对于适当的治疗至关重要.
- 生物标志物发现对于区分这些条件至关重要.
研究的目的:
- 确定潜在的蛋白质生物标志物,以区分胸膜囊和胸膜瘤.
- 为了比较胸膜囊和胸膜瘤亚型之间的蛋白质表达特征.
- 使用生物信息学工具验证候选生物标志物.
主要方法:
- 在患者样本上使用TMT标记和UPLC-MS进行蛋白质组分析 (胸膜囊,胸膜瘤B1,胸膜瘤B3).
- 生物信息分析包括基因本体学 (GO),KEGG通路和蛋白质-蛋白质相互作用网络.
- 使用GEPIA和UALCAN数据库验证差异表达基因 (DEGs).
主要成果:
- 在B1与MTC比较中发现了49个DEP,在B3与MTC比较中发现了27个DEP,在B3与B1比较中发现了38个DEP.
- 几种蛋白质,包括LGALS3BP,与胸膜囊相比,在胸膜瘤中被持续改变.
- GEPIA的分析证实了在胸腺瘤患者中LGALS3BP的显著上调.
结论:
- LGALS3BP是一种有前途的生物标志物,用于区分胸腺瘤和胸腺囊.
- 蛋白质基因分析提供了关于胸膜病变之间的分子差异的见解.
- 需要进一步验证,以确定LGALS3BP作为临床诊断工具.
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