通过浸泡发酵将果皮生物转化为伊塔康酸,并通过响应表面方法学对参数进行统计优化
Shagufta Saeed1, Sibtain Ahmed2, Fatima Qureshi1
1University of Veterinary and Animal Sciences, Lahore, Punjab, Pakistan.
PeerJ
|October 22, 2024
概括
果皮是一种水果副产品,可以作为一种廉价的基质,通过与黑色阿斯伯菌的发酵生产伊塔康酸 (IA). 这项研究优化了条件,以实现高纯度的4.6g/LIA生产.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物化学工程 生物化学工程
背景情况:
- 伊塔康酸 (IA) 是一种重要的有机酸,具有多种工业应用.
- 高的基质成本,主要是纯葡萄糖,阻碍了高效的IA生产.
- 果皮是一种丰富的,富含糖的,未充分利用的农业工业副产品.
研究的目的:
- 调查使用果皮作为itaconic酸生物合成基质的可行性.
- 优化物理化学参数,以最大限度地提高阿斯伯吉卢斯尼格尔菌的伊塔科尼酸产量.
- 为了确定纯度和产量,从果皮水解液中生产的伊塔科尼酸.
主要方法:
- 使用Aspergillus niger的果皮水解剂的浸泡发酵.
- 使用响应表面方法 (RSM) 与中央复合设计 (CCD) 优化发酵参数 (化时间,温度,动,注射剂大小,pH).
- 使用溶剂提取 (n-butanol) 提取和净化伊塔康酸,并通过富里埃变换红外光谱 (FTIR) 和高性能液态染色学 (HPLC) 进行分析.
主要成果:
- 在优化条件下,达到了4.6g/L的最大伊塔克酸度.
- 最佳参数包括10% (w/v) 的果皮水解剂,3毫升的注射剂,5天的潜伏期,pH为3,32°C,200rpm的动.
- 使用FTIR和HPLC成功识别和量化了高纯度伊塔康酸 (98.74%).
结论:
- 果皮是一种有前途且具有成本效益的基质,用于itaconic酸的生物合成.
- 该研究表明,农业工业废弃物利用有价值的化学品生产的潜力.
- 建议在生物反应器规模上进行进一步的优化,以便在工业规模生产伊塔康酸.
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