长距离转录因子结合位点集群区域可能通过早期人类胚胎的相位分离相互作用调节转录调节
Mengge Tian1,2, Xiaohan Tang1, Zhangyi Ouyang2
1The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Computational and structural biotechnology journal
|October 22, 2024
概括
我们在早期人类胚胎发育中确定了转录因子结合位 (TFBS) 和它们的集群区域 (TFCR). TFCR复杂性与胚胎基因组激活后的基因表达相关,这表明它在发育中的作用.
科学领域:
- 发展生物学 发展生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 细胞类型特定的基因表达对哺乳动物胚胎发育至关重要.
- 基因调节元件 (CREs) 和转录因子 (TFs) 调节基因表达.
- 转录因子结合位 (TFBS) 是调节基因组功能的关键指标.
研究的目的:
- 在早期人类胚胎发育过程中识别和量化TFBS和转录因子结合位点-集群区域 (TFCR).
- 通过量化复杂度 (TC) 测量来评估TFCR的功能意义.
- 探索TFCR复杂性和基因表达后异位基因组激活之间的关系.
主要方法:
- 在早期人类胚胎发育的五个阶段对染色质可访问性数据的分析.
- 识别和量化TFBS和TFCRs.
- 计算TFCR复杂性 (TC) 来评估监管要素的功能.
主要成果:
- 在早期人类胚胎发育过程中,TFBS和TFCR被确定并量化.
- 从8细胞阶段开始,在TFCR复杂性和基因表达之间观察到强烈的相关性.
- 定义了远程TFCRs (LR-TFCRs),它们可能通过相分离来调节基因表达.
结论:
- TFCR复杂性是人类早期发育过程中调节元件功能的定量衡量.
- TFCR复杂性与在胚胎基因组激活后的基因表达相关.
- 在早期胚胎发生过程中,LR-TFCRs可能通过相分离在基因调节中发挥作用.
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