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从阿尔及利亚特贝萨市的新鲜蔬菜中产生ESBL和AMPC的肠杆菌
Amel Amra1, Manel Debabza1, Raoudha Dziri2,3
1Bioactive Molecules and Applications Laboratory, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University (E.C.L.T.U.), Tebessa, Algeria.
概括
阿尔及利亚特贝萨的蔬菜被产生ESBL或AmpC的多药耐药肠道细菌污染. 这项研究发现了新的ESBL基因变异,突出了对新鲜食品安全的公共卫生问题.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 食品传播病原体出现抗菌素耐药性的出现是全球健康威胁.
- 产生扩展光谱β-乳酸酶 (ESBL) 和AmpC酶的肠杆菌是感染的重要原因.
- 新鲜产品可以作为将耐药细菌传播给消费者的载体.
研究的目的:
- 评估新鲜蔬菜和水果受到ESBLs产生肠道细菌 (ESBLs-E) 和AMpC产生肠道细菌的污染水平.
- 为了表征在已识别的分离物中存在的ESBL基因.
- 为了确定这些耐药细菌在阿尔及利亚Tebessa的流行率.
主要方法:
- 采集和微生物分析132个新鲜农产品样本 (67种蔬菜,65种水果).
- 隔离和识别第三代抗素的肠道细菌.
- 使用双磁盘协同作用和AmpC磁盘测试检测ESBL和AmpC生产的表型.
- 通过PCR和测序来对ESBL基因进行分子表征.
主要成果:
- 从19.40%的蔬菜样本中找到了16个耐药的肠道细菌分离物;没有检测到水果污染.
- 所有的分离物体都表现出多种药物耐药性. 确认有14个菌株是ESBL生产者 (包括Klebsiella spp.,Kluyvera spp.,Enterobacter cloacae),还有2个是AmpC生产者 (Citrobacter freundii,E. cloacae).
- 在七个菌株中,PCR检测出了ESBL基因,主要是blaCTX-M. 测序揭示了阿尔及利亚的新型ESBL基因变异,包括blaCTX-M-79,blaCTX-M-107,blaCTX-M-117,blaTEM-112,blaTEM-125,blaTEM-194和blaSHV-176.
结论:
- 阿尔及利亚Tebessa的新鲜蔬菜是多种耐药ESBLs-E和AMPC产生的肠杆菌的潜在污染来源.
- 鉴定出新的ESBL基因变异突显了加强监测和控制措施的必要性.
- 调查结果强调了食品安全实践的重要性,以减轻通过新鲜产品传播抗菌素耐药性的风险.
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