CD169+巨细胞通过Keap1/Nrf2/HO-1轴调解过敏性鼻炎的免疫反应
Wenwen Qi1, Chengcheng Liu2,3, Lei Shi1
1Department of Otolaryngology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 22, 2024
概括
CD169+巨细胞增加过敏性鼻炎 (AR) 鼻组织,通过Keap1/Nrf2/HO-1通路促进氨酸和活性氧物种 (ROS) 的产生,影响AR中的树突细胞功能.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- CD169+巨细胞是参与抗原呈现和免疫耐受性的独特巨细胞子集.
- CD169+巨细胞在过敏性鼻炎 (AR) 中的特定作用在很大程度上仍未被定义.
研究的目的:
- 研究CD169+巨细胞在过敏性鼻炎 (AR) 背景下的特征和作用.
- 阐明CD169+巨细胞影响AR中的免疫反应的分子机制.
主要方法:
- 在人类AR鼻腔粘膜中分析CD169+巨体表达.
- 建立和比较对AR的CD169转基因小鼠模型.
- 评估CD169对免疫细胞 (乙氨基,Th细胞,Treg细胞) 和树突细胞 (DC) 迁移的淘汰效应.
- 代谢分析以确定涉及的分子途径.
主要成果:
- 在AR患者的鼻腔粘膜中观察到CD169+巨细胞的显著升高.
- 发现CD169+巨细胞可上调氨酸的产生,并增加活性氧物种 (ROS) 水平.
- 确定Keap1/Nrf2/HO-1信号通路和SLC38A2是CD169+巨细胞功能中的关键调解者.
- 在AR模型中,CD169+巨细胞会影响乙素,Th细胞,Treg细胞和树突细胞迁移.
结论:
- CD169+巨细胞在过敏性鼻炎的发病过程中发挥着重要作用.
- 通过Keap1/Nrf2/HO-1轴介导的CD169+巨细胞对氨酸生产和ROS水平的升级对AR至关重要.
- CD169+巨细胞对于通过SLC38A2促进树突细胞的氨酸吸收至关重要,这突显了它们在AR免疫反应中的重要性.
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