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相关概念视频

RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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Improving Translational Accuracy02:07

Improving Translational Accuracy

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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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Base Excision Repair01:54

Base Excision Repair

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相关实验视频

Updated: Jun 9, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

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精确转录组编辑 精确转录组编辑

Crystal Chen1, Lei S Qi2,3,4

  • 1Department of Chemical Engineering, Stanford University, Stanford, California 94305, United States.

ACS synthetic biology
|October 22, 2024
PubMed
概括
此摘要是机器生成的。

精确的转录组工程为控制哺乳动物细胞中的基因表达提供了新的方法. 这些RNA编辑工具可以改变转录的丰富性,翻译等,为未来的疗法显示出希望.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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相关实验视频

Last Updated: Jun 9, 2025

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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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科学领域:

  • 分子生物学分子生物学
  • 基因表达规范 基因表达规范
  • 在RNA治疗方面,RNA疗法.

背景情况:

  • RNA操纵是控制哺乳动物细胞基因表达的关键.
  • 最近的进展集中在精确的转录组编辑工具上.
  • 这些工具针对各种修改的特定RNA转录.

研究的目的:

  • 审查最近在精密转录组编辑方面的进展.
  • 要突出工程RNA转录的工具.
  • 讨论这些技术在研究和治疗方面的潜力.

主要方法:

  • 关于转录组工程工具的当前文献的审查.
  • 专注于改变RNA丰度,翻译,基编辑,异形和化学修改的方法.
  • 在细胞和体内模型中评估效率.

主要成果:

  • 已经开发了几种精确的转录组工程工具.
  • 这些工具使RNA转录的精确修改成为可能.
  • 在一些方法的相关细胞和体内模型中证明了效率.

结论:

  • 精确转录基因工程是一个快速发展的领域.
  • 需要进一步的研究来确保临床安全性和疗效.
  • 具有RNA生物学研究和基因/细胞治疗的巨大潜力.