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在Hyriopsis cumingii中对TGF-β I型受体基因的克隆和表达分析

Linying Liu¹, Peipei Yi², Chengyi Jiang¹

¹Life Science College, Nanchang University, Nanchang 330031, China.

Zoological science

|October 22, 2024

概括

淡水中的TGF-β型I受体 (TGF-βRI) 基因对于伤口愈合和免疫反应至关重要. 它的表达在受伤和细菌刺激后增加,这表明它在修复过程中的作用.

科学领域:

* 分子生物学 * 分子生物学
* 免疫学免疫学
* * 海洋生物学海洋生物学

背景情况:

*转化生长因子-β (TGF-β) 信号通路对于伤口愈合和免疫调节至关重要.
*了解TGF-βRI在无脊椎动物 (如淡水) 中的作用,可以了解保存的生物机制.

研究的目的:

* 克隆和表征来自淡水 Hyriopsis cumingii 的 TGF-β I 型受体 (TGF-βRI) 基因.
* 为了研究TGF-β RI的表达模式,以应对免疫挑战和创伤.
* 阐明TGF-β RI在鱼的伤口修复和免疫反应中的参与.

主要方法:

*基因克隆和测序以获得TGF-β RI的全长cDNA.
*实时定量PCR (RT-qPCR) 分析各种组织中的基因表达和刺激后的基因表达.
* 复合TGF-βRI蛋白的体外表达和用于蛋白质分析的西方涂抹.
* 用SB431542抑制剂治疗,以评估途径的参与.

主要成果:

* TGF-β RI基因被确定为2017 bp的全长cDNA,编码517个氨基酸,具有不同的功能域.
*在所有健康的肌肉组织中检测到TGF-β RI基因表达,在用Aeromonas hydrophila和peptidoglycan刺激后,在血细胞和肝胰腺中显著上调.
*TGF-β RI的mRNA和蛋白质水平在创伤后增加,与伤口愈合标志物 (TGF-β,Smad5,MMP1/19,TIMP1/2) 的表达相关,并在SB431542治疗下降.

结论:

*从Hyriopsis cumingii中克隆的TGF-β RI表现出保存的结构特征.
*TGF-β RI在淡水的免疫反应和伤口愈合过程中发挥着重要作用.
*这项研究提供了分子证据,证明TGF-β信号通路参与无脊椎动物的防御和修复机制.

关键词:

这种植物是Hyriopsis cumingii.这就是TGF-βRI.克隆,就是克隆人类.免疫力是一种免疫力.伤口修复伤口修复

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