在 Bacillus subtilis 生物膜中,yfj 操作子的差异表达
James P Finn1,2, Cora Luzinski2, Briana M Burton2
1Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Applied and environmental microbiology
|October 22, 2024
概括
在Bacillus subtilis生物膜中的第七类分泌系统 (T7SS) 基质是通过分泌细胞表达的. 这项研究确定了yfj操作子.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 分子生物学分子生物学
背景情况:
- 第七类蛋白质分泌系统 (T7SS) 对病原体生存和微生物竞争至关重要.
- 细菌细菌生物膜表现出生理异质性,不同的细胞命运影响基因表达.
- 生物膜内T7SS基质的表达模式在很大程度上是未知的.
研究的目的:
- 为了确定Bacillus subtilis生物膜中哪些特定的细胞类型表达T7SS基质.
- 调查yfj操作子的调节,该操作子编码一个潜在的T7SS基质.
主要方法:
- 在yfj操作子促进区的in silico分析中,以确定调节序列.
- 构建一个yfj促进体-报告体融合以研究基因表达.
- 在 degU 和 spo0A 删除突变体中对基因表达的分析.
- 在生物膜细胞中微观观察yfj记者表达.
主要成果:
- DegU积极调节yfj操作子的表达,删除取消表达.
- 在yfj操作调节上,Spo0A对 DegU 有相反的作用.
- 显微镜检测显示,在生物膜内的胞细胞中,yfj操作子的特异表达.
结论:
- 进入分泌的细胞是表达Bacillus subtilis中yfj操作子产物的主要亚群.
- 这一发现表明,T7SS在社区相关活动中发挥了作用,例如生物电影中的食人行为.
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