把垃圾变成宝藏:Hermetia illucens微生物群和工业侧流的生物降解
Patrick Klüber1, Friscasari F Gurusinga1,2, Sabine Hurka1,2,3
1Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, Germany.
Applied and environmental microbiology
|October 22, 2024
概括
黑士兵幼虫 (BSFL) 可以食用高纤维的饮食,但会变小. 它们的肠道微生物,特别是Bacillaceae,有助于消化坚硬的植物材料,使BSFL能够将废物转化为有价值的生物质.
科学领域:
- 昆虫生物学 昆虫生物学
- 微生物学 微生物学
- 废物管理 废物管理
背景情况:
- 有机副流由于大量和处置问题而带来了环境挑战.
- 黑兵幼虫 (BSFL) 通过将有机废物再循环转化为有价值的生物质,提供了一个可持续的解决方案.
- 植物性侧流通常含有难以消化的纤维和反营养化合物.
研究的目的:
- 研究高纤维饮食对黑士兵幼虫 (BSFL) 生长的影响.
- 为了分析BSFL的肠道微生物群落的变化,他们吃了不同的饮食.
- 了解肠道微生物组在BSFL处理纤维基质的能力中的作用.
主要方法:
- 在高纤维饮食中养的BSFL:空果束 (EFB),土豆肉质 (PP) 和棉压蛋糕 (CPC),以料 (CF) 作为对照.
- 监测了幼虫生长表现 (大小,存活率).
- 使用一种依赖种植的方法来隔离和识别BSFL的肠道细菌.
- 使用in silico和in vitro方法 (刚果红色试验) 来评估被分离的细菌降解的纤维素.
主要成果:
- 在EFB,PP和CPC养的幼虫的生长率有所下降,但存活率很高 (>90%).
- 总共有329种细菌菌株被分离出来;在食EFB的幼虫中,Bacillaceae占主导地位.
- 巴西拉氏菌群分离物在和体外表现出纤维素降解能力.
- 在食高蛋白饮食 (CPC,CF) 的幼虫中,Enterobacteriaceae和Morganellaceae是普遍存在的.
结论:
- 肠道微生物组对于BSFL消化有机纤维物质至关重要,使其能够在低价值基板上完成生命周期.
- BSFL有效地将工业侧流转化为有价值的生物质,有助于减少废物和可持续性.
- 饮食成分影响BSFL的肠道微生物社区结构,影响其消化能力.
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