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塑料纳米颗粒干扰了初级星体细胞中的细胞外囊路径

Kamil Adamiak¹, Marta Sidoryk-Węgrzynowicz¹, Beata Dąbrowska-Bouta¹

¹Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Institute Polish Academy of Sciences, 5 Pawińskiego str., Warsaw 02-106, Poland.

Ecotoxicology and environmental safety

|October 22, 2024

概括

聚乙烯纳米粒子 (PS-NP) 进入星体细胞,并通过细胞外囊泡 (EV) 释放出来,可能传播毒性. 这种细胞间转移会影响星体细胞的通信和蛋白质分类机制.

科学领域:

环境毒理学环境毒理学
细胞生物学细胞生物学
纳米技术纳米技术

背景情况:

塑料微粒 (MP) 和纳米粒子 (NP) 导致环境污染,并对健康构成风险.
纳米粒子毒性机制,包括氧化应激和细胞死亡,需要进一步研究.
星体细胞在大脑功能中起着至关重要的作用,是纳米粒子毒性的潜在目标.

研究的目的:

研究聚乙烯纳米颗粒 (PS-NP) 在初级星球细胞中的内化和细胞内命运.
确定PS-NPs是否会干扰通过细胞外囊泡 (EVs) 介导的细胞间通信.
分析PS-NP对恒星细胞释放的EV蛋白质载荷的影响.

主要方法:

初级天体细胞培养暴露于光PS-NP在不同度和时间点.
电子显微镜和共聚焦成像被用来跟踪PS-NP内部化和局部化.
蛋白质组分析是在暴露的星体细胞释放的细胞外囊泡 (EV) 上进行的.

主要成果:

星体细胞内部化了PS-NP,并以度依赖的方式在内体-溶体系统内的细胞质中积累.
暴露于PS-NPs的星体细胞释放含有封装PS-NPs的EV,促进细胞间传输.
PS-NPs改变了释放的EV的蛋白质组成,降低了CD47,CSTB和CNDP2的水平.

结论:

星球细胞中PS-NP的细胞间传输是由EVs介导的.
通过EV介导的PS-NP释放可能会调节单个细胞中的毒性,但可以将影响扩散到邻近的细胞.
暴露于PS-NP会破坏EV中的蛋白质分类,可能会损害星细胞介导的细胞间通信.

关键词:

电动汽车 (EV) 是一个电动汽车是蛋白质基的内体-解体系统内体-解体系统.纳米毒理学研究聚乙烯纳米粒子聚乙烯纳米粒子

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