Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Highly efficient and low-mosaicism <i>piggyBac</i> transgenesis platform for rapid founder phenotyping.

iScience·2026
Same author

A Delphi survey of patients and clinicians on a unified and stratified approach to managing GCA-PMR spectrum disease.

Rheumatology (Oxford, England)·2026
Same author

A conceptual framework for a RA probability score integrating classification criteria and point-of-care ultrasound in early arthritis.

Rheumatology (Oxford, England)·2026
Same author

Phase 3 Trial of Secukinumab in Polymyalgia Rheumatica.

The New England journal of medicine·2026
Same author

A national survey report to facilitate rheumatology ultrasound curriculum proposal.

Rheumatology (Oxford, England)·2026
Same author

A Unified, Stratified Approach to the Large Vessel Vasculitis Spectrum and Imaging Implications.

International journal of rheumatic diseases·2026

相关实验视频

Updated: Jun 9, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

10.9K

m6ATM:一种深度学习框架,用于用纳米孔长时间读取的RNA序列数据去神秘化m6A表写体.

Boyi Yu1, Genta Nagae2, Yutaka Midorikawa3

  • 1Advanced Data Science Division, Research Center of Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku 153-8904, Tokyo, Japan.

Briefings in bioinformatics
|October 22, 2024
PubMed
概括

在信使RNA中绘制N6-甲基氨酸 (m6A) 映射对于理解它在疾病中的作用至关重要. 一个名为m6ATM的新工具使用直接RNA测序数据的深度学习来准确检测m6A位点,从而推进表观转录学研究.

关键词:
深度神经网络是一个神经网络.标志性转录组学 标志性转录组学长时间读取的RNA测序.m6A 一个很好的.

更多相关视频

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.2K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.0K

相关实验视频

Last Updated: Jun 9, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

10.9K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.2K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.0K

科学领域:

  • 史诗转录组学 史诗转录组学
  • 计算生物学 计算生物学
  • 基因组学就是基因组学.

背景情况:

  • N6-甲基氨酸 (m6A) 是一种关键的RNA修饰,参与生物过程和疾病.
  • 准确的全转录组m6A分析对于研究至关重要.
  • 直接RNA测序 (DRS) 显示了RNA修饰检测的潜力,但数据解释具有挑战性.

研究的目的:

  • 使用DRS数据开发一个计算管道,用于精确的m6A位点检测.
  • 在单基分辨率下对m6A表谱组进行表征.

主要方法:

  • 介绍了基于Python的m6A转录组全方位映射器 (m6ATM),一个基于Python的管道.
  • 深度神经网络的应用,包括WaveNet编码器和双流多实例学习.
  • 使用牛津纳米孔技术直接RNA测序 (DRS) 数据.

主要成果:

  • m6ATM在体外数据集上实现了高精度 (80%-98%).
  • 在与人类细胞系数据的基准测试中表现优于现有的工具.
  • 证明能够提供固体测量信息,并确定PEG10作为肝癌中潜在的m6A标.

结论:

  • m6ATM是用于m6A检测的高性能工具.
  • 该工具有助于进步的表皮转录组研究.
  • 能够精确地绘制m6A修饰的疾病相关研究.