一种类型A响应调节器等同对的表达和功能分歧,在Populus deltoides进化过程中通过分散的重复形成
Jing Lu1, Ying-Nan Chen2, Tong-Ming Yin1
1State Key Laboratory for Tree Genetics and Breeding, Nanjing Forestry University, Nanjing, 210037, China.
Communications biology
|October 22, 2024
概括
植物中的基因重复推动了进化. 在树中,PdRR8和PdFERR起源于一个共同的祖先,但PdRR8是功能性正义词,在细胞因子信号传递中显示出不同的表达和作用.
科学领域:
- 植物进化生物学 植物进化生物学
- 分子遗传学 分子遗传学
- 植物激素信号传递器
背景情况:
- 基因重复和分歧是植物进化的关键驱动力.
- 参与细胞因素信号传递的阿拉比多普西斯A型反应调节器 (ARR) 家族,显示了广泛的基因扩张.
- 在木质植物中了解A型ARR同类物是有限的.
研究的目的:
- 调查A型ARR基因家族在红和单种植物中的进化史.
- 在Populus deltoides (树) 中识别和描述独特的A型ARR基因对.
- 确定重复基因的功能作用和进化关系.
主要方法:
- 对A型ARR基因家族的家族遗传学分析.
- 在Populus deltoides (PdRR8和PdFERR) 中分散的基因重复的识别和表征.
- 在各种刺激下 (寒冷,6-BA) 基因表达分析和Arabidopsis突变物中的功能补充.
主要成果:
- 在Populus deltoides中发现了一对独特的分散重复物,PdRR8和PdFERR,它们起源于rosaids的共同祖先.
- PdRR8被保留在花中,而PdFERR对应物被丢失;PdRR8在叶子中表达很高,PdFERR在雌性花中表达.
- PdRR8补充了Arabidopsis突变体,表明它是ARR16-ARR17的真正正义体,而PdFERR未能挽救缺陷,表明功能分歧.
结论:
- 尽管PdRR8和PdFERR起源于共同的祖先,并且在负选择下演变,但它们表现出差异性表达和功能分歧.
- PdRR8是细胞激素信号通路中ARR16-ARR17对应物的功能正义体.
- 这项研究提供了关于植物基因家族的进化和功能多样化的见解.
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