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相关概念视频

Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

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Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Transcription Initiation01:47

Transcription Initiation

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Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
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相关实验视频

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Spt5编排了加密的转录抑制和转录方向性.

Haejin An1, Hyeokjun Yang1, Daeyoup Lee2

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Communications biology
|October 22, 2024
PubMed
概括

Spt5蛋白抑制了内基抗感应转录,保持了准确的RNA聚合酶II转录. 它的耗尽改变了染色质结构,增加了反意义RNA并影响了基因表达调节.

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 基因规则 基因规则
  • 染色体生物学 染色体生物学

背景情况:

  • Spt5是一种保守的转录因子,调节基因表达的多个阶段.
  • 之前的研究指出,在Spt5突变时,促进体附近的反感应转录增加.
  • 在反感转录调节中Spt5的确切作用尚不清楚.

研究的目的:

  • 在酵母中识别和表征Spt5p受限制的内基因反感转录.
  • 研究Spt5调节反意义转录的机制.
  • 为了确定Spt5-介导的反感抑制的功能意义.

主要方法:

  • 对酵母细胞中具有不同Spt5功能状态的反意义转录的分析.
  • 调查Spt5 CTR酸化在反意义调节中的作用.
  • 在Spt5耗尽时评估染色质结构变化 (基因素乙化).
  • 在Spt5的反意义限制中涉及终止因素的识别.

主要成果:

  • Spt5p强烈抑制了内基因反感应转录,特别是对于具有高感应转录的基因.
  • Spt5 CTR 酸化对于其反意义调节功能至关重要.
  • 由于Spt5的枯竭,导致基因素乙化增加,改变染色质并启动反感应转录.
  • 在人类基因中观察到的Spt5-介导的反感觉抑制的保存.

结论:

  • 在平衡转录双向性方面,Spt5通过抑制不稳定转录 (DUTs) 发挥了新的作用.
  • 通过Spt5媒介抑制反感应转录对于准确的RNA聚合酶II转录至关重要.
  • 了解Spt5在反意义调节中的作用,可以了解如何保持基因组稳定性和适当的基因表达.