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使用Corynebacterium crenatum的代谢工程,从曼尼托和葡萄糖中增强L-氨酸的产生

Gang Yang¹, Sicheng Xiong¹, Mingzhu Huang¹

¹School of Life Sciences, School of Health, Jiangxi Normal University, Nanchang, 330022, China.

Microbial cell factories

|October 22, 2024

概括

通过使用Corynebacterium crenatum.获得了有效的微生物生产L-氨酸 (L-Tyr). 这项研究引入了曼尼托尔作为一种新的碳来源,在可持续的生物制造平台上显著提高了L-Tyr的产量.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物合成微生物合成

背景情况:

氨酸 (L-Tyr) 是一种重要的芳香氨基酸,市场需求日益增长.
传统的L-Tyr生产方法面临着局限性,引发了对微生物合成的兴趣.
开发可持续和高效的生物生产路线至关重要.

研究的目的:

为了改造Corynebacterium crenatum以提高L-Tyrosine (L-Tyr) 的产量.
探索曼尼托尔作为L-Tyr生物合成的可持续碳来源的使用.
为了优化代谢途径以增加L-Tyr产量.

主要方法:

代谢工程策略包括途径消除和基因过度表达 (aroB,aroD,aroE,tyrA).
通过淘汰mtlR和过度表达mtlD和pfkB以利用曼尼来对C.crenatum进行遗传修饰.
优化发酵条件,包括葡萄糖与曼尼托尔的比例.

主要成果:

从葡萄糖-曼尼托混合物中获得6.42g/L的L-Tyr产量,比单独的葡萄糖增加了16.9%.
通过料批发发酵,提高L-轮胎产量至34.6g/L.
在C. glutamicum中表现出报告中最高的L-Tyr标位.

结论:

率先使用曼尼托尔作为微生物L-Tyr生物合成的碳来源.
阐明了曼尼托相关基因在微生物代谢中对L-Tyr生产的作用.
建立了一个有前途的非食品竞争平台,用于L-Tyr和其他目标化合物生物合成.

关键词:

科里尼细菌 (Corynebacterium crenatum) 是一种细菌.在L-tyrosine的使用中.曼尼托尔 (Mannitol) 是一种有毒的药物.混合碳来源混合碳来源

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