相关实验视频

Updated: Jun 9, 2025

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

Published on: September 16, 2022

来自慢性伤口的多药耐药性葡萄球杆菌 (Staphylococcus epidermidis) 在人类伤口模型中损害了愈合

Miroslav Dinić^1,2, Rebecca Verpile¹, Jamie L Burgess¹

¹Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

|October 23, 2024

概括

在静脉腿 (VLU) 中的 Staphylococcus epidermidis 可以形成生物膜,阻碍伤口愈合. 这些细菌诱导炎症并表现出抗菌素耐药性,有助于慢性的发展.

科学领域:

微生物学微生物学
伤口治愈研究研究伤口治愈研究
皮肤病学皮肤病学

背景情况:

静脉性腿部 (VLU) 是常见的慢性伤口,具有破坏的微生物群落.
常见的皮肤共生菌Staphylococcus epidermidis可以通过形成生物膜来阻碍VLU的愈合.

研究的目的:

为了调查VLU中的Staphylococcus epidermidis的分布和毒性.
分析从VLU中分离的S. epidermidis的抗菌耐药性和生物膜形成能力.

主要方法:

整个基因组测序S. epidermidis的分离物.
抗微生物敏感性测试和体外生物膜测试.
活体人类伤口模型,以评估促炎潜力和愈合影响.

主要成果:

从VLU中分离出来的S. epidermidis可以通过生物膜诱导的IL-1β,IL-8和IL-6抑制再表皮化.
高细胞外矩阵结合能力与抗微生物耐药性和特定基因表达 (embp,sdrG) 相相关.
由VLU衍生的S. epidermidis表现出致病特征,影响伤口愈合.

结论:

在VLU中的S. epidermidis具有致病特征,阻碍愈合.
耐治疗,毒性S. epidermidis血统的出现是慢性患者的一个担忧.

关键词:

感染感染的感染.角质细胞 (keratinocytes) 是一种细胞.翻译研究是翻译研究.静脉腿部静脉腿部伤口愈合伤口愈合

更多相关视频

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation

Published on: June 16, 2023

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...