第六类分泌系统在蜜蜂肠共生体中促进了物种内竞争和宿主相互作用
Erick V S Motta1,2, Patrick J Lariviere1,3, Korin R Jones1
1Department of Integrative Biology, The University of Texas at Austin, TX 78712.
概括
蜜蜂肠道共生体Snodgrassella alvi的第六类分泌系统 (T6SS) 对于物种内竞争和塑造宿主免疫反应至关重要,尽管对于肠道殖民并不重要.
科学领域:
- 微生物学 微生物学
- 昆虫微生物学 昆虫微生物学
- 同生相互作用的交互.
背景情况:
- 六型分泌系统 (T6SS) 是一种细菌武器,用于细胞间和细胞内传递因子.
- 斯诺德格拉塞拉 (Snodgrassella alvi) 是蜜蜂肠道中的一个关键共生体,影响宿主健康.
- 了解T6SS在S. alvi中的作用对于蜜蜂肠道微生物组研究至关重要.
研究的目的:
- 研究T6SS-1和T6SS-2在Snodgrassella alvi wkB2.2.中的功能.
- 确定T6SSs对S. alvi殖民,竞争和蜜蜂宿主免疫调节的影响.
主要方法:
- 在S. alvi wkB2.2.中产生T6SS基因淘汰突变 (tssD,tssE)
- 在蜂蜜蜂的肠道中进行体内殖民和竞争分析.
- 蜜蜂肠道组织的转录基因分析.
主要成果:
- 对于S. alvi殖民来说,T6SS不是必不可少的,但T6SS-2会影响殖民水平.
- T6SS-1调解了S. alvi菌株之间的种内竞争.
- S. alvi T6SSs调节蜜蜂免疫基因表达,包括抗菌.
结论:
- 在S. alvi中,T6SS对蜂蜜蜂肠道微生物组内的竞争相互作用很重要.
- T6SSs有助于S. alvi和蜜蜂宿主免疫系统之间的复杂相互作用.
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