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Gene Expression Analyses in Human Follicles
Published on: February 17, 2023
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在原始毛囊激活过程中,WNT驱动的通路和颗粒状细胞-卵细胞相互作用的转录学签名
Hinako M Takase1, Tappei Mishina1,2, Tetsutaro Hayashi3,4
1Laboratory for Chromosome Segregation, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe, Japan.
PloS one
|October 23, 2024
概括
卵巢颗粒细胞 (GCs) 中WNT信号干扰损害了卵细胞的发育,导致不孕. 这突显了原始毛囊激活 (PFA) 和女性生殖的关键细胞间通信.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 原始卵泡激活 (PFA) 对于女性生育至关重要,涉及卵细胞和粒状细胞 (GCs) 的协调变化.
- 在PFA期间控制同步休眠和激活的精确分子机制仍然不完全理解.
研究的目的:
- 通过使用Wntless (Wls) 条件淘汰 (cKO) 小鼠,研究WNT信号在PFA期间调节GC-oocyte交叉声中的作用.
- 为了阐明在没有WNT信号传递的情况下,在PFA之后GC和卵细胞的转录变化.
主要方法:
- 在Wls cKO和对照小鼠的卵巢上进行了全面的单细胞RNA测序 (scRNA-seq).
- 分析的重点是GC和卵细胞的转录基因变化,亚种群识别和途径分析.
主要成果:
- Wls cKO小鼠表现出破坏的WNT信号,影响了GC成熟,并导致PFA后GC转录基因轨迹的分歧.
- 卵细胞显示了适度的转录变化,但GC中的WNT信号损失甚至在PFA之前就影响了卵细胞基因表达.
- Wls cKO小鼠的不孕症与受损的GC-卵细胞交叉和改变的卵细胞微环境有关.
结论:
- WNT信号传递对于GC成熟至关重要,并且对于在PFA期间保持适当的GC-oocyte分子交叉来说至关重要.
- 干扰WNT信号传递对卵泡发育有深远的影响,这强调了细胞间通信对女性生殖成功的重要性.
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