通过Wnt/β-catenin信号传递引起的真实环境PM2.5诱导的角膜上皮质屏障破坏
Jiayin Sun1, Yanting Li2, Haowen Chen1
1Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266071, China.
Ecotoxicology and environmental safety
|October 23, 2024
概括
暴露于细颗粒物 (PM2.5) 会损害角膜上皮屏障,导致眼睛表面疾病. 这项研究揭示PM2.5通过激活Wnt/β-catenin通路来破坏角膜细胞,从而损害眼睛的健康.
科学领域:
- 眼科医生 眼科 眼科
- 环境健康 环境健康
- 细胞生物学 细胞生物学
背景情况:
- 暴露于细颗粒物 (PM2.5) 与眼睛表面疾病的风险增加有关.
- 真实环境PM2.5影响角膜上皮质屏障功能的具体机制尚不清楚.
研究的目的:
- 研究真实环境PM2.5对角膜上皮质屏障功能及其潜在机制的影响.
- 阐明Wnt/β-catenin信号通路在PM2.5引起的角膜损伤中的作用.
主要方法:
- 暴露C57BL/6J小鼠在现实环境中的PM2.5 (平均度为140.18μg/m3) 4周和8周.
- 显微镜检查角膜上皮细胞的排列,微和层完整性.
- 在实验室分析PM2.5对Wnt/β-catenin通路激活和角膜上皮细胞中E-cadherin和ZO-1的表达的影响.
主要成果:
- 暴露于PM2.5导致角膜上皮细胞失调,微细胞丧失,细胞层连接中断.
- 在体外,100微克/毫升PM2.5激活了Wnt/β-catenin信号通路.
- 暴露于PM2.5显著降低了E-cadherin (1.81倍) 和ZO-1 (2.25倍) 的表达,损害了屏障功能.
结论:
- 持续暴露于现实环境中的PM2.5会损害角膜上皮质屏障的功能.
- Wnt/β-catenin信号通路在PM2.5诱导的角膜上皮屏障功能障碍中起着至关重要的作用.
- 研究结果强调了空气污染对眼睛健康的有害影响,并为保护策略提供了基础.
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