研究表型,上腺转录组和产卵的血清新陈代谢之间的相关性,这些产卵在相同的压力因素下表现出不同的行为
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 430064, PR China.
在压力下的产卵子中,高活动性避开会降低体重增加和卵产量. 这种行为也会损害免疫和抗氧化反应,与特定的基因和代谢物变化有关.
科学领域:
- 动物科学动物科学
- 行为生物学 行为生物学
- 基因组学就是基因组学.
背景情况:
- 在子环境中放置子会经历诸如新奇恐惧之类的压力因素,影响行为和生产力.
- 在相同的压力条件下子行为的变化尚未完全理解.
- 了解行为反应对于改善子福利和产量至关重要.
研究的目的:
- 为了研究不同的避开行为如何影响子产的生产性能,免疫反应,抗氧化能力,上腺基因表达和血清代谢物概况.
- 确定关键的生物过程,基因和与压力下的行为变化相关的代谢物.
- 为了全面理解,将表型,遗传和代谢数据关联起来.
主要方法:
- 选择了42周大的产卵 (N=300),并根据行为测试将24只分为高活动避开 (HAA) 和低活动避开 (LAA) 组.
- 在60天内进行了表型,遗传 (上腺转录组) 和代谢分析.
- 利用生物信息学来丰富和综合分析多学科数据.
主要成果:
- 与LAA组相比,HAA组的体重增加 (ΔW) 和平均每日蛋重 (ADEW) 显着较低,料与蛋的比例较高 (P<0.05).
- 在HAA中,血清免疫球蛋白A,总抗氧化能力,超氧化失调酶和催化酶 (CAT) 活性降低,血清ACTH水平较高 (P<0.05).
- 上腺转录组分析揭示了148个差异表达的基因,在神经活性联体受体相互作用和氧化酸化中具有显著的途径丰富;11个代谢物在组之间存在差异.
结论:
- 在压力下产的行为回避策略显著影响生产效率和生理状态.
- 每天平均蛋重的差异可能与 gabapentin 和 EXOSC3 基因表达的变化有关,影响血清 CAT 水平.
- 这项研究提供了对家禽对压力的行为反应背后的分子机制的见解,为改善动物福利和生产力提供了策略.
更多相关视频
06:39Using a Murine Model of Psychosocial Stress in Pregnancy as a Translationally Relevant Paradigm for Psychiatric Disorders in Mothers and Infants
Published on: June 13, 2021
09:06A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
Published on: October 24, 2018
相关概念视频
Hormones of the Adrenal Glands
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
Hypothalamic-Pituitary Axis
Physiological Foundation of Stress
Role of the Sympathetic Nervous System
Adrenaline triggers the...
Anatomy of the Adrenal Glands
These glands possess a distinctive yellow tinge due to the stored cholesterol and fatty acids required for hormone synthesis. They are encased in a fibrous capsule and cushioned by fat.
The adrenal gland comprises two distinct...
Adrenal Gland Disorders
Adrenal insufficiency, characterized by insufficient cortisol and aldosterone production, leads to conditions like Addison's disease. This disorder, affecting the adrenal cortex, exhibits symptoms such as skin bronzing, dehydration, low blood pressure, fatigue, and weight loss. Congenital adrenal hyperplasia, a genetic ailment causing...
Introduction to Stress and Lifestyle
