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相关概念视频

Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

5.6K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
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Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.6K
Spindle Assembly02:50

Spindle Assembly

3.6K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.6K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.5K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.5K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
The Mitotic Spindle02:27

The Mitotic Spindle

6.4K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
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相关实验视频

Updated: Jun 9, 2025

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

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戈尔金45通过其核局部化序列协助线粒分裂.

Jingkai Gao1, Lianhui Zhu1, Xihua Yue1

  • 1School of Life Science and Technology, ShanghaiTech University, Shanghai, China.

Biochemical and biophysical research communications
|October 23, 2024
PubMed
概括

戈尔金45在线粒分裂过程中将激酶PLK1招募到激素细胞中. 这种由RanGTP调节的Golgin45-PLK1相互作用对于适当的细胞分裂至关重要,并防止了线粒细胞的停止.

关键词:
在Grasp5555中,你会发现一个很好的方法.戈尔吉戈尔吉是一座古老的城市.这就是Golgin4545的原因.进口中的β2在KNL1上.在Kinetochore中进行演出.线粒分裂 (mitosis) 是一种发生在细胞间的过程.PLK1 PLK1 的意思是

更多相关视频

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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相关实验视频

Last Updated: Jun 9, 2025

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 戈尔吉器官在细胞分裂的线粒分裂过程中碎片.
  • 戈尔吉蛋白调节了戈尔吉的拆卸/重组和线形成.
  • 戈尔吉蛋白在其他线粒体事件中的作用尚不清楚.

研究的目的:

  • 为了研究Golgin45的作用,一个Golgi绑定蛋白质,在线粒分裂.
  • 阐明Golgin45影响线粒细胞进展的机制.

主要方法:

  • 研究了Golgin45-PLK1在线粒分裂期间的相互作用.
  • 利用Golgin45中的R375A突变来破坏Golgin45-importin β2相互作用.
  • 分析了PLK1局部化到kinetochores和线粒细胞的进展.

主要成果:

  • 戈尔金45通过与importin β2和KNL1.1的相互作用将PLK1招募到kinetochores中.
  • 在kinetochores中的RanGTP调节了Golgin45-PLK1复合物的释放.
  • 干扰Golgin45-importin β2相互作用导致PLK1局部受损和线粒性停止.

结论:

  • 戈尔金45在中介Ran-依赖的PLK1丰富中发挥着新的作用.
  • 这种机制对于线粒分裂的正确进展至关重要.
  • 戈尔吉45是一种关键的调节器的线粒体事件超出戈尔吉的动态.