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碳量子点的毒性综合评估:表面电荷的影响

Pei-Luen Jiang¹, Yan-Yu Hong¹, Lingyan Yang²

¹Department of Biotechnology, National Formosa University, Yunlin, 63208, Taiwan.

|October 23, 2024

概括

表面电荷会影响碳量子点 (CQD) 的安全性. 带正电荷的CQD显示出细胞毒性,但总体而言,CQD显示出良好的生物医学安全性,特别是保持时间最小化.

科学领域:

纳米技术纳米技术
生物医学工程生物医学工程
材料科学材料科学材料科学

背景情况:

碳量子点 (CQDs) 显示出各种应用的前景.
评估CQD的生物医学安全性对于其临床转化至关重要.
不同的表面修饰可以改变CQD的生物相互作用和毒性.

研究的目的:

评估具有不同表面修饰的CQDs的生物医学安全和健康风险.
研究CQD表面电荷及其细胞毒性作用之间的关系.
评估CQD在生物医学中安全应用的潜力.

主要方法:

三种类型的CQD的合成和表征:基于酸 (CQDs-A),基于精三化物 (CQDs-S) 和组合 (CQDs-A/S).
使用HEK293细胞进行体外细胞毒性测定,氧化应激分析和炎症反应评估 (NLRP3炎症体).
在BALB/c小鼠体内研究,包括器官损伤评估和病理评估.

主要成果:

所有的CQD都小于10nm;正电荷的CQDs-S和CQDs-A/S在体外表现出显著的细胞毒性和诱导氧化应激.
没有观察到NLRP3炎症酶的激活,这表明炎症反应有限.
在体内研究证实了CQD的安全性,即使高剂量也没有观察到器官损伤或病理.

结论:

CQD的表面电荷是影响其细胞毒性作用和氧化应激诱导的关键因素.
CQD显示出相当大的生物医学安全性,脏完整性得到维护.
尽量减少CQD的保留时间是提高其在生物医学应用中的安全性的关键.

关键词:

碳量子点是一个量子点.细胞毒性细胞毒性毒性毒性毒性氧化反应反应氧化反应表面的电荷来自表面的电荷.

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