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从Lavandula angustifolia中低温提取多糖的超声波辅助. :优化,结构特征和抗炎活性

Lei Cao¹, Wei Liu¹, Ha Jing²

¹Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China.

International journal of biological macromolecules

|October 23, 2024

概括

来自Lavandula angustifolia的多糖化合物. 通过减少细胞和斑马鱼模型中的关键炎症标志物,证明了显著的抗炎作用. 这些发现支持它们在功能性食品和药品中的潜在用途.

科学领域:

药理学药理学是指药理学的学科.
自然产品化学自然产品化学
免疫学免疫学免疫学

背景情况:

拉文杜拉格斯蒂福利亚.米尔. (普通衣草) 是一种具有显著药理价值的中国传统药用草本.
多糖因其多样化的生物活动而闻名,包括免疫调节性质.
了解L. angustifolia多糖的抗炎作用可能会导致新的治疗应用.

研究的目的:

为了优化L. angustifolia的提取. 使用低温超声辅助提取的多糖.
为了隔离,净化和表征提取的多糖.
评估分离的多糖的体外和体内抗炎活性.

主要方法:

响应表面方法 (RSM) 用于提取优化.
DEAE-650M和SuperdexTM 200染色学用于隔离和净化.
部分酸性水解,甲基化和2DNMR光谱用于LAPS1.1的结构阐明.
在体外测定使用LPS刺激的RAW264.7巨细胞来测量炎症性细胞因子 (IL-1β,IL-6,TNF) 和氧化 (NO) 生产.
在体内斑马鱼模型,以评估中性粒细胞迁移在CuSO4刺激的反应.

主要成果:

优化条件产生了两个同聚糖,LAPW1 (33.6 kDa) 和LAPS1 (95.9 kDa).
确定LAPS1的主要结构为具有特定重复单元的异多糖.
无论是LAPW1还是LAPS1,在LPS刺激的RAW264.7细胞中都显著降低了炎症性细胞因子 (IL-1β,IL-6,TNF) 和氧化的产生.
在体内研究显示斑马鱼中中性粒细胞迁移显著减少,证实了抗炎活性.

结论:

这项研究成功地优化了从L. angustifolia Mill.中提取和表征抗炎多糖的方法.
LAPW1和LAPS1表现出强大的抗炎性质,可以通过减少炎症介质和中性粒细胞迁移来证明.
这些发现表明,L. angustifolia Mill. 聚糖对功能性食品和用于炎症疾病的药品的开发充满希望.

关键词:

2D NMR 是一个二维的NMR.芳香植物的多糖化物.欧洲爱好者斑马鱼的炎症模型

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