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在产后自发分娩之前,外围白细胞迁移的激活
Han Lee1, Ai Takamizu2, Yuji Nishizaki3
1Department of Physiology, University of Alberta, Edmonton, Canada.
American journal of obstetrics and gynecology
|October 23, 2024
概括
人类胎儿膜释放化学吸引剂,增加白细胞迁移近期. 这种白细胞迁移试验可以高精度地预测7天内分娩.
科学领域:
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
- 孕产妇和胎儿的医学
背景情况:
- 白细胞迁移到子宫对于分娩至关重要.
- 人类胎儿膜释放时间依赖的化学吸引剂.
- 这些信号激活白细胞进行分娩.
研究的目的:
- 从怀孕晚期到产后,跟踪白细胞迁移-反应的变化.
- 在分娩前分析每周的迁移变化.
- 与迁移和血清细胞因子/化学因子水平相关联化学因子受体表达.
- 评估胎儿膜条件介质和母体血清对白细胞响应性的影响.
- 评估白细胞迁移试验,以预测在一周内分娩.
主要方法:
- 修改的博伊登室用于白细胞迁移.
- 流细胞计用于细胞表型评估.
- 对于化学因子受体表达的定量PCR.
- 对血清细胞因子/化学因子进行多重分析.
- 生物测试用于评估条件介质和血清.
- 为了预测测试性能,ROC和成本曲线分析.
主要成果:
- 白细胞迁移在预产时增加,然后在分娩后减少.
- 在分娩前1-2周观察到的峰值迁移增加.
- 白细胞中化学因受体mRNA的增加与增强的迁移相关.
- 劳动开始时血清中细胞因子和化学因子升高.
- 胎儿膜介质和母体血清提高了白细胞的反应能力.
- 该测试预测了在7天内分娩,具有高正预测值 (96.1%).
结论:
- 长期人类胎儿膜释放化学吸引剂,随着怀孕的进展,这些化学吸引剂逐渐激活和吸引白细胞.
- 白细胞迁移试验显示了预测即将到来的分娩的强大潜力.
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