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Victoria F Miari¹, Matthew R Blakiston², Priya Solanki²

¹Department of Infection Biology, London School of Hygiene & Tropical Medicine Faculty of Infectious and Tropical Diseases, London, UK victoria.miari@lshtm.ac.uk.

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|October 23, 2024

概括

患有多种淋病感染的患者中,很大一部分患者可能携带不同抗微生物药物耐药性的不同菌株. 这一发现影响了对尼塞利亚淋病菌的测试,监测和治疗策略.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

尼塞利亚淋病 (Neisseria gonorrhoeae) 引起淋病,这是一个日益严重的全球健康问题,原因是抗菌素耐药性 (AMR) 的增加.
多部位感染很常见,一些患者在不同的解剖部位携带不同的菌株.
这些不同的菌株可能具有不同的抗微生物敏感性概况,使治疗复杂化.

研究的目的:

为了估计多个尼塞利亚淋病菌株在患有多部位感染的患者中的患病率.
使用抗微生物敏感性测试 (AST) 个人资料和分子方法来区分菌株.
为了将基因型和表型抗微生物耐药性相关联.

主要方法:

从患有多部位感染的患者中分离的淋巴球菌的横截面研究.
确定关键抗微生物药物的最小抑制度 (MIC).
全基因组测序 (WGS) 用于菌株类型 (NG-MAST,NG-STAR,MLST,SNP基因组) 和AMR基因鉴定.

主要成果:

对41名患者的91个分离物的分析显示,有6名患者 (14.6%) 具有不一致的MIC.
在这6名患者中,WGS在4名患者中证实了明显的冠状病毒菌株.
基于WGS的AMR预测与表型AST数据有很好的相关性.

结论:

显著比例的多部位性淋病感染涉及多个菌株与不同的AST配置文件.
这凸显了需要仔细抽取患者样本和量身定制的敏感性测试.
这些发现对淋病监测和优化抗生素治疗有意义.

关键词:

临床实验室技术临床实验室技术药物耐药性,细菌的耐药性尼塞利亚淋病 (Neisseria gonorrhoeae) 是一种疾病.

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