相关实验视频

Updated: Jun 9, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

充分利用1.0毫米识别器的潜力在基于UHPLC-HRMS的非向代谢学列中

Danila La Gioia^1,2, Emanuela Salviati¹, Manuela Giovanna Basilicata³

¹Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 84084, Fisciano, SA, Italy.

Analytical and bioanalytical chemistry

|October 23, 2024

概括

一个1.0毫米的微孔柱设置显著提高了使用UHPLC-HRMS在非目标代谢学中的灵敏度和特征检测. 这种方法可以改善各种样本类型的低度代谢物分析和数据质量.

科学领域:

分析化学分析化学
代谢学代谢学代谢学
质谱测量质量谱测量

背景情况:

使用超高性能液态染色学-高分辨率质谱学 (UHPLC-HRMS) 的非目标代谢学通常使用2.1毫米内径 (i.d.) 这是柱子. 柱子.
挑战包括分析代谢组的广泛度范围和有限的样本量.
减少柱子直径被探索为解决方案,以提高分析性能.

研究的目的:

为了评估1.0毫米的IDD的性能. 微孔UHPLC-HRMS设置与标准的2.1mm i.d.相比. 系统用于非目标代谢学.
在各种样本类型和分析模式中评估灵敏度,检测极限 (LOD),量化极限 (LOQ),可重复性和特征检测.

主要方法:

对比1.0毫米的I.D. 微孔和2.1毫米的I.D. 微型UHPLC和标准UHPLC系统上的标准钻孔柱,分别与四极轨道 HRMS 合.
对极性标准代谢物,血,干血斑 (DBS) 和结直肠癌患者衍生器官 (PDO) 的分析.
使用了逆相 (RP) 和水友交互液态染色学 (HILIC) 模式,以及完全扫描数据依赖采集 (FS-DDA).

主要成果:

在1.0毫米的ID.D. 这种设置显示了平均3.8倍的灵敏度增长,与2.1mm i.d.相比,平均LOD (1.48 ng/mL vs. 6.18 ng/mL) 和LOQ (4.94 ng/mL vs. 20.60 ng/mL) 显着较低. 一个设置.设置.设置.
使用微孔方法检测和量化了所有代谢物在量化的下限 (LLOQ),在保留时间 (0.29% vs. 0.63%) 和峰值区域 (4.65% vs. 7.27%) 的较低变化系数 (CV%) 中显示出更好的重复性.
在各种样本类型和模式中,观察到的MS1特征 (38.95%),MS2特征 (39.26%) 和MS/MS光谱匹配 (18.23%) 的显著增加.

结论:

在1.0毫米的ID.D. UHPLC-HRMS配置为增强分析覆盖率提供了一个可行的策略,特别是对于数量有限的样品.
这种微孔方法保持了与标准的2.1mm i.d.相比的分析吞吐量和稳定性. 在减少溶剂消耗的同时,格式化.
这些发现支持采用1.0毫米的IDD. 列为改善非目标代谢表现的列.

关键词:

质谱测量质量谱测量代谢学代谢学代谢学微型钻孔是如何形成的在UHPLC中,UHPLC是UHPLC.没有目标,没有目标.

更多相关视频

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

相关概念视频

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...