开发了一种P磁共振光谱技术,以在3T时量化NADH和NAD+
Julian Mevenkamp1,2, Yvonne M H Bruls1,2, Rodrigo Mancilla2,3
1Maastricht University Medical Center, Department of Radiology & Nuclear Medicine, Maastricht, The Netherlands.
Nature communications
|October 23, 2024
概括
研究人员开发了一种使用31P-MRS测量人类肌肉中NADH和NAD+的新型非侵入性方法. 这种技术揭示了这些代谢物的差异,与身体活动和代谢健康有关.
科学领域:
- 生物化学 生物化学
- 生理学 生理学 生理学
- 医疗成像医学成像
背景情况:
- 尼古丁胺胺二核酸 (NAD+) 和其减少形式 (NADH) 是关键的氧化还原因子,参与能量代谢.
- NAD+在刺激线粒体生物发生和维持代谢健康方面发挥着至关重要的作用.
- 在人类骨肌肉中非侵入性地定量NADH和NAD+是具有挑战性的,因为信号重叠,特别是与α-adenosine三酸盐 (α-ATP).
研究的目的:
- 开发和验证一种新的非侵入性磁共振光谱 (31P-MRS) 方法,用于量化人类骨肌肉中的NADH和NAD+.
- 评估开发的31P-MRS技术的可复制性和生理相关性.
- 将31P-MRS测量与活检分析进行比较,并调查与体力活动相关的代谢差异.
主要方法:
- 开发一种新的MR序列,使用双旋回声与修改的z-Filter相结合,用于强大的α-ATP信号抑制.
- 在临床3TMRI扫描仪上应用31P-MRS序列,以在体内定量NADH和NAD+.
- 在幽灵和人类实验对象的验证,包括可重现性评估,缺血引起的代谢物变化,以及与肌肉活检数据的比较.
主要成果:
- 新的MR序列有效地抑制了重叠的α-ATP信号,使NAD+和NADH的精确量化成为可能.
- 该方法在人类骨肌肉中表现出良好的可重复性.
- 在缺血期间检测到NAD+的生理下降和NADH的增加,与预期的代谢转变一致.
- 较高的NAD+和较低的NADH水平被观察到身体活跃的老年人与久坐的人相比.
结论:
- 开发的31P-MRS技术提供了一种可靠的,非侵入性的方法来量化人类骨肌中的NADH和NAD+.
- 这种方法可以检测与生理挑战相关的代谢变化,如缺血和生活方式因素,如身体活动.
- 研究结果表明,NAD+水平与代谢健康有关,并且可能受到老年人身体活动的影响.
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